State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou, China.
Insect Mol Biol. 2010 Apr;19(2):243-52. doi: 10.1111/j.1365-2583.2009.00983.x. Epub 2010 Jan 18.
Activated protein 4 (AP-4), an E-box DNA-binding protein, was cloned from the cotton bollworm, Helicoverpa armigera (Har). The expression of Har-AP-4 mRNA and the protein that it encodes are significantly higher in nondiapause pupae than in diapause pupae. In vitro-translated Har-AP-4 can bind specifically to the E-box motif on the promoter of the diapause hormone and pheromone biosynthesis-activating neuropeptide (DH-PBAN). Har-AP-4, fused with the green fluorescent protein (GFP), is localized to the nucleus, and overexpression of Har-AP-4 can significantly activate the promoter of the DH-PBAN gene that is involved in nondiapause pupal development in H. armigera. These results suggest that Har-AP-4, which binds to the promoter of DH-PBAN, may play a role in regulating pupal development in H. armigera.
激活蛋白 4（AP-4）是一种 E 盒 DNA 结合蛋白，从棉铃虫（Helicoverpa armigera）中克隆得到。Har-AP-4mRNA 的表达及其编码的蛋白质在非滞育蛹中显著高于滞育蛹。体外翻译的 Har-AP-4 可以特异性地结合到滞育激素和性信息素生物合成激活神经肽（DH-PBAN）启动子上的 E 盒基序。Har-AP-4 与绿色荧光蛋白（GFP）融合后定位于细胞核，过表达 Har-AP-4 可以显著激活参与非滞育蛹发育的 DH-PBAN 基因的启动子。这些结果表明，结合 DH-PBAN 启动子的 Har-AP-4 可能在调节棉铃虫蛹发育中发挥作用。
