Integrated Clinic, Araçatuba Dental School, UNESP, São Paulo, Brazil.
Dent Traumatol. 2010 Feb;26(1):80-5. doi: 10.1111/j.1600-9657.2009.00856.x.
The maintenance of the avulsed teeth in appropriate media for preserving the cellular viability has been important for repairing the periodontal ligament and preventing the root resorption after tooth reimplantation. Propolis is a substance capable of preserving cellular viability. This study aimed to analyze the propolis substance as a storage media for maintaining the avulsed teeth, besides to determine the ideal time period for keeping the tooth inside it. Thus, 60 maxillary right central incisors of rats were extracted and divided into five groups. In groups I and II, teeth were kept in propolis for 60 min and 6 h, respectively; in group III, teeth were kept in milk for 6 h; in group IV, teeth were kept dry for 60 min; and in group V, they were immediately reimplanted. All teeth had their root canals filled with calcium hydroxide paste. Following, teeth were reimplanted in their sockets. After 15 and 60 days, animals were killed and the obtained samples were processed in laboratory for microscopic and morphometric analyzing. The results showed that the occurrence of inflammatory resorption, dental ankylosis and the formation of the connective tissue parallel to the root surface were similar among groups. It could be verified a greater occurrence of replacement resorption in group IV when comparing to other groups. In groups I and IV, the presence of periodontal ligament-like connective tissue was substantially smaller than the other groups. Regarding to the cementum amount over the root, it could be observed that this was present in smaller amount in groups I and IV. Group II was similar to groups III and IV. Therefore, according to the results of this study, the use of propolis as a storage media for maintaining avulsed teeth could be highlighted, and the 6-h period was more appropriate than the 60-min period.
将脱位的牙齿保存在适当的介质中以维持细胞活力对于修复牙周韧带和防止牙再植入后的牙根吸收非常重要。蜂胶是一种能够维持细胞活力的物质。本研究旨在分析蜂胶物质作为储存介质，以保持脱位的牙齿，并确定将牙齿保存在其中的理想时间。因此，从 60 只雄性大鼠的上颌右中切牙中提取并分为五组。在第 I 组和第 II 组中，牙齿分别保存在蜂胶中 60 分钟和 6 小时；在第 III 组中，牙齿保存在牛奶中 6 小时；在第 IV 组中，牙齿干燥 60 分钟；在第 V 组中，牙齿立即再植入。所有牙齿的根管均用氢氧化钙糊剂填充。然后，将牙齿再植入其牙槽窝中。在第 15 天和第 60 天，处死动物，将获得的样本在实验室中进行处理，进行显微镜和形态计量分析。结果表明，各组之间炎症性吸收、牙骨粘连和与牙根表面平行的结缔组织形成相似。与其他组相比，第 IV 组的替代吸收发生率更高。在第 I 组和第 IV 组中，牙周韧带样结缔组织的存在明显小于其他组。关于根表面上的牙骨质量，可以观察到第 I 组和第 IV 组的牙骨质量较少。第 II 组与第 III 组和第 IV 组相似。因此，根据本研究的结果，可以突出使用蜂胶作为保持脱位牙齿的储存介质，并且 6 小时的时间比 60 分钟的时间更合适。
