Bryan Heather M, Sim Kathrin A, Darimont Chris T, Paquet Paul C, Wagner Brent, Muñoz-Fuentes Violeta, Smits Judit E, Chilton Neil B
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Dr, Saskatoon, Saskatchewan S7N 5B4, Canada.
J Wildl Dis. 2010 Jan;46(1):297-302. doi: 10.7589/0090-3558-46.1.297.
First-stage nematode larvae with a dorsal-spine (DSL) were detected in five of 1,565 fecal samples from gray wolves (Canis lupus) collected in British Columbia, Canada, between 2005 and 2008. Molecular techniques were used to identify the DSL because it was not possible to determine their species identity using morphologic characters. The DSL were identified as Parelaphostrongylus odocoilei based on the results of single-strand conformation polymorphism (SSCP) analyses and DNA sequencing of the ribosomal DNA first and second internal transcribed spacers. Finding DSL of P. odocoilei in the feces of gray wolves was unexpected because P. odocoilei adults are parasites of cervids and bovids. The most likely explanation for the presence of DSL in wolf feces is that they were ingested along with the viscera of recently consumed prey. This was probably black-tailed deer (Odocoileus hemionus columbianus), which are known in the sampling area to be hosts of P. odocoilei. The present study demonstrates the use of SSCP and DNA sequencing for the identification, to the species level, of parasitic nematode larvae in feces.
2005年至2008年期间，在加拿大不列颠哥伦比亚省采集的1565份灰狼（Canis lupus）粪便样本中，有5份检测到带有背刺（DSL）的第一阶段线虫幼虫。由于无法通过形态特征确定其物种身份，因此使用分子技术来鉴定DSL。根据单链构象多态性（SSCP）分析结果以及核糖体DNA第一和第二内部转录间隔区的DNA测序，DSL被鉴定为奥氏副裸头线虫（Parelaphostrongylus odocoilei）。在灰狼粪便中发现奥氏副裸头线虫的DSL出乎意料，因为奥氏副裸头线虫成虫是鹿科动物和牛科动物的寄生虫。狼粪便中存在DSL最可能的解释是，它们与最近食用猎物的内脏一起被摄入。这很可能是黑尾鹿（Odocoileus hemionus columbianus），在采样区域已知其是奥氏副裸头线虫的宿主。本研究证明了使用SSCP和DNA测序在物种水平上鉴定粪便中寄生线虫幼虫的方法。
