Turakulov Ia Kh, Atakhanova B A
Probl Endokrinol (Mosk). 1977 Sep-Oct;23(5):68-72.
A study was made of cyclic guanosine-3+, 5+-monophosphate (cGMP) on protein synthesis in the cell-free system of the thyroid gland. Preincubation of S30 fraction with cGMP led to increase of labeled amino acid incorporation into proteins (by 56% with the cGMP concentration of 1.73 X 10(-5) M, and by 160% with the concentration of 2 X 10(-4)). No stimulating effect was observed on addition of cGMP directly into the incubation medium. The results obtained suggested that protein synthesis was stimulated under the effect of cGMP at the translation level. The same protein fractions [27S, 19S, 12S, 3-8S (I) and 3-8S (II)] were revealed by disc-electrophoresis of soluble proteins synthesized in the cell-free system in which polyribosomes preincubated with cAMP (1.73 X 10(-5) M) were used as in the system devoid of cAMP. Specific radioactivity of thyroglobulin subunits corresponded in experiments with cAMP to that in experiments without cAMP. At the same time specific radioactivity of 19S thyroglobulin was more than doubled. It can be supposed that thyroglobulin subunit synthesis was accelerated under the effect of cAMP, and, as a result, labeled thyroglobulin increased in the system. The data obtained confirmed a hypothesis put forward earlier that cAMP had a direct influence upon the translation process.
对环鸟苷-3′,5′-单磷酸（cGMP）在甲状腺无细胞系统中蛋白质合成的作用进行了研究。用cGMP对S30组分进行预孵育，导致标记氨基酸掺入蛋白质的量增加（当cGMP浓度为1.73×10⁻⁵M时增加56%，当浓度为2×10⁻⁴时增加160%）。将cGMP直接加入孵育培养基中未观察到刺激作用。所得结果表明，cGMP在翻译水平上刺激了蛋白质合成。通过圆盘电泳分析无细胞系统中合成的可溶性蛋白质，发现与使用预孵育有cAMP（1.73×10⁻⁵M）的多核糖体的无cAMP系统中相同的蛋白质组分[27S、19S、12S、3 - 8S（I）和3 - 8S（II）]。在有cAMP的实验中，甲状腺球蛋白亚基的比放射性与无cAMP的实验中相当。同时，19S甲状腺球蛋白的比放射性增加了一倍多。可以推测，在cAMP的作用下甲状腺球蛋白亚基的合成加速，结果系统中标记的甲状腺球蛋白增加。所得数据证实了先前提出的cAMP对翻译过程有直接影响的假设。
