Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy and Tooth Regeneration, Capital Medical University School of Stomatology, Beijing, China.
Cells Tissues Organs. 2010;191(5):357-64. doi: 10.1159/000276589. Epub 2010 Jan 14.
The use of allogeneic stem cells strongly extends the range of stem cell applications in dentistry; however, immunological rejection remains a major concern. There is little information about the immunological features of dental-related stem cells in the literature. Therefore, we investigated the immunological characteristics of stem cells from the root apical papilla (SCAP) of swine in vitro by measuring T cell immunomodulation and apoptosis. We found that SCAP expressed a low level of swine leukocyte antigen (SLA) class I molecules and were negative for SLA class II DR molecules. Moreover, SCAP could inhibit autologous T cell proliferation stimulated by phytohemagglutinin (PHA) and a one-way mixed lymphocyte reaction in a dose-dependent manner. In addition, SCAP could suppress proliferation of allogeneic T cells in a dose-dependent manner, with or without mitomycin C pretreatment. Moreover, soluble factor(s) may be involved in the SCAP-mediated immune suppression. After a 5-day coculture of SCAP, allogeneic T cells, and PHA, only 1.22% of T cells were apoptotic. These data indicated that SCAP were weakly immunogenic and suppressed T cell proliferation in vitro through an apoptosis-independent mechanism.
同种异体干细胞的使用大大扩展了干细胞在牙科领域的应用范围；然而，免疫排斥仍然是一个主要关注点。文献中关于与牙齿相关的干细胞的免疫学特征的信息很少。因此，我们通过测量 T 细胞免疫调节和细胞凋亡来研究猪根尖乳头干细胞（SCAP）的免疫学特征。我们发现 SCAP 表达低水平的猪白细胞抗原（SLA）I 类分子，并且 SLA 类 II DR 分子呈阴性。此外，SCAP 可以剂量依赖性方式抑制由植物血凝素（PHA）和单向混合淋巴细胞反应刺激的自身 T 细胞增殖。此外，SCAP 可以在有或没有丝裂霉素 C 预处理的情况下，剂量依赖性地抑制同种异体 T 细胞的增殖。此外，可溶性因子（s）可能参与了 SCAP 介导的免疫抑制。在 SCAP、同种异体 T 细胞和 PHA 的 5 天共培养后，只有 1.22%的 T 细胞发生凋亡。这些数据表明 SCAP 的免疫原性较弱，并且通过非凋亡依赖的机制在体外抑制 T 细胞增殖。
