半合成细胞色素c。
Semisynthetic cytochrome c.
作者信息
Barstow L E, Young R S, Yakali E, Sharp J J, O'Brien J C, Berman P W, Harbury H A
出版信息
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4248-50. doi: 10.1073/pnas.74.10.4248.
Abstract
Horse heart cytochrome c can be split with cyanogen bromide into a heme peptide (residues 1-65) and a nonheme peptide (residues 66-104). In a process involving (i) complex formation between the two fragments and (ii) restoration of the severed peptide linkage, a fully active cytochrome c preparation can be re-formed. Use has been made of this process to couple the heme peptide to peptide 66-104 synthesized by the Merrifield solid-phase procedure. The semisynthetic product formed in this manner is indistinguishable from reconstituted cytochrome c prepared with nonsynthetic peptide 66-104.
摘要
马心脏细胞色素c可用溴化氰裂解成一个血红素肽（第1至65位氨基酸残基）和一个非血红素肽（第66至104位氨基酸残基）。在一个涉及（i）两个片段之间形成复合物以及（ii）恢复断裂的肽键的过程中，可以重新形成一个完全活性的细胞色素c制剂。利用这个过程将血红素肽与通过梅里菲尔德固相法合成的66 - 104肽进行偶联。以这种方式形成的半合成产物与用非合成的66 - 104肽重构的细胞色素c无法区分。
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Studies on cytochrome c. XIV. Synthesis of the protected heptadecapeptide (sequence 88-104) of horse heart cytochrome c.细胞色素c的研究。十四。马心细胞色素c的受保护十七肽（序列88 - 104）的合成。
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引用本文的文献
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The semisynthesis of fragments corresponding to residues 66-104 of horse heart cytochrome c.马心脏细胞色素c中对应于66 - 104位残基片段的半合成。
Biochem J. 1979 Apr 1;179(1):169-82. doi: 10.1042/bj1790169.
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Semisynthetic horse heart [65-homoserine]cytochrome c from three fragments.来自三个片段的半合成马心脏[65-高丝氨酸]细胞色素c。
Proc Natl Acad Sci U S A. 1979 Jan;76(1):61-5. doi: 10.1073/pnas.76.1.61.
本文引用的文献
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The reversible removal of cytochrome c from mitochondria.细胞色素c从线粒体的可逆性移除。
J Biol Chem. 1960 Feb;235:531-4.
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A quantitative immunochemical measure of the primary interaction between I BSA and antibody.一种对牛血清白蛋白（BSA）与抗体之间主要相互作用的定量免疫化学测定方法。
J Infect Dis. 1958 Nov-Dec;103(3):239-62. doi: 10.1093/infdis/103.3.239.
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Use of hydrogen fluoride in Merrifield solid-phase peptide synthesis.氟化氢在梅里菲尔德固相肽合成中的应用。
J Am Chem Soc. 1967 Jan 4;89(1):181-2. doi: 10.1021/ja00977a057.
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Cleavage of cytochrome c with cyanogen bromide.用溴化氰裂解细胞色素c。
Biochim Biophys Acta. 1970 Dec 22;221(3):489-96. doi: 10.1016/0005-2795(70)90219-9.
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Reconstitution of horse heart cytochrome c: interaction of the components obtained upon cleavage of the peptide bond following methionine residue 65.马心脏细胞色素c的重构：甲硫氨酸残基65之后肽键断裂所获得的各组分之间的相互作用。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3036-9. doi: 10.1073/pnas.68.12.3036.
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Complex formation between methionine and a heme peptide from cytochrome c.蛋氨酸与细胞色素c中的血红素肽之间的复合物形成。
Proc Natl Acad Sci U S A. 1965 Dec;54(6):1658-64. doi: 10.1073/pnas.54.6.1658.
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Use of lactoperoxidase catalyzed iodination in immunochemical studies.乳过氧化物酶催化碘化反应在免疫化学研究中的应用。
Immunochemistry. 1971 Mar;8(3):289-97. doi: 10.1016/0019-2791(71)90484-8.
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