Boon P J, Tesser G I
Int J Pept Protein Res. 1985 May;25(5):510-6. doi: 10.1111/j.1399-3011.1985.tb02204.x.
The last step in the semisynthesis of horse cytochrome c analogues (formation of the bond 65-66) requires the conformation of the complex between two complementary fragments, (1-65) lactone and (66-104). The fragments can be obtained from a limited degradation with cyanogen bromide. The amino component in this reaction can also be obtained from organo chemical synthesis in which the C-terminal fragment (81-104) is required in a selectively protected form. The latter is available from a cyanogen bromide degradation of ubiquitously protected cytochrome c. The details of the protection/deprotection reaction and the properties of nonadecamethylsulfonylethyloxycarbonyl cytochrome c are described.
马细胞色素c类似物半合成的最后一步（形成65-66键）需要两个互补片段（1-65）内酯和（66-104）之间复合物的构象。这些片段可通过溴化氰的有限降解获得。该反应中的氨基组分也可通过有机化学合成获得，其中C末端片段（81-104）需要以选择性保护的形式存在。后者可通过对普遍保护的细胞色素c进行溴化氰降解获得。描述了保护/脱保护反应的细节以及十九甲基磺酰基乙氧基羰基细胞色素c的性质。
