通过 PCR-RFLP 评估兰蜂 Euglossa fimbriata（膜翅目：蜜蜂科）种群的线粒体 DNA 多样性。

Mitochondrial DNA diversity of orchid bee Euglossa fimbriata (Hymenoptera: Apidae) populations assessed by PCR-RFLP.

机构信息

Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR 86051-990, Brazil.

出版信息

Biochem Genet. 2010 Apr;48(3-4):326-41. doi: 10.1007/s10528-009-9325-4. Epub 2010 Jan 21.

DOI:10.1007/s10528-009-9325-4

PMID:20091342

Abstract

Euglossa fimbriata is a euglossine species widely distributed in Brazil and occurring primarily in Atlantic Forest remnants. In this study, the genetic mitochondrial structure of E. fimbriata from six Atlantic Forest fragments was studied by RFLP analysis of three PCR-amplified mtDNA gene segments (16S, COI-COII, and cyt b). Ten composite haplotypes were identified, six of which were exclusive and represented singleton mitotypes. Low haplotype diversity (0.085-0.289) and nucleotide diversity (0.000-0.002) were detected within samples. AMOVA partitioned 91.13% of the overall genetic variation within samples and 8.87% (phi(st) = 0.089; P < 0.05) among samples. Pairwise comparisons indicated high levels of differentiation among some pairs of samples (phi(st) = 0.161-0.218; P < 0.05). These high levels indicate that these populations of E. fimbriata, despite their highly fragmented landscape, apparently have not suffered loss of genetic variation, suggesting that this particular population is not currently endangered.

摘要

褶裳凤蝶是广泛分布于巴西的凤蝶科物种，主要存在于大西洋森林的残余地区。在这项研究中，通过对三个 PCR 扩增的线粒体 DNA 基因片段（16S、COI-COII 和 cyt b）的 RFLP 分析，研究了来自六个大西洋森林片段的褶裳凤蝶的遗传线粒体结构。共鉴定出 10 种复合单倍型，其中 6 种是独特的，代表单一的单倍型。在样本中检测到低的单倍型多样性（0.085-0.289）和核苷酸多样性（0.000-0.002）。AMOVA 将总体遗传变异的 91.13%分配给样本内，8.87%（phi(st) = 0.089；P < 0.05）分配给样本间。成对比较表明，一些样本对之间存在高度的分化（phi(st) = 0.161-0.218；P < 0.05）。这些高水平表明，尽管这些褶裳凤蝶种群的景观高度破碎，但显然没有遗传变异的丧失，这表明该特定种群目前尚未受到威胁。