Songram Onuma, Sittipraneed Siriporn, Klinbunga Sirawut
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10300, Thailand.
Biochem Genet. 2006 Jun;44(5-6):256-69. doi: 10.1007/s10528-006-9030-5. Epub 2006 Sep 20.
Genetic diversity of the honeybee (Apis cerana) in Thailand collected from north, northeast, the central region, peninsular Thailand, and Samui Island (n = 181) was examined by PCR-RFLP of ATPase6-ATPase8. Interestingly, 78 individuals (43.09%) of the southern-latitude bees exhibited length heteroplasmy of the PCR product. The gel-eluted ATPase6-ATPase8 (825 bp) of each bee was restricted with TaqI, SspI, and VspI, respectively. Eight mitotypes were generated and revealed biogeographic differentiation between conspecific samples of A. cerana. AAA, ACA, AAD, BAA, ADA, and ABA were found only in the north-to-central samples (north, northeast, and central region); BBB and BBC were found in the southern-latitude bees; and BBC was restrictively found in the Samui sample. Large genetic distances were observed between each of the north-to-central samples and peninsular Thailand and Samui samples, but lower levels of genetic distance were found within each region. Geographic heterogeneity and phylogenetic analyses indicated that Thai A. cerana could be genetically differentiated into northern Thailand, peninsular Thailand, and Samui Island populations.
通过ATPase6 - ATPase8的PCR - RFLP技术，对从泰国北部、东北部、中部地区、泰国半岛以及苏梅岛采集的181只蜜蜂（中华蜜蜂）的遗传多样性进行了检测。有趣的是，78只（43.09%）来自低纬度地区的蜜蜂表现出PCR产物的长度异质性。分别用TaqI、SspI和VspI对每只蜜蜂凝胶洗脱的ATPase6 - ATPase8（825 bp）进行酶切。共产生了8种单倍型，揭示了中华蜜蜂同种种群之间的生物地理分化。AAA、ACA、AAD、BAA、ADA和ABA仅在北部至中部样本（北部、东北部和中部地区）中发现；BBB和BBC在低纬度地区的蜜蜂中发现；而BBC仅在苏梅岛样本中发现。在每个北部至中部样本与泰国半岛和苏梅岛样本之间观察到较大的遗传距离，但在每个区域内发现的遗传距离水平较低。地理异质性和系统发育分析表明，泰国的中华蜜蜂在基因上可分为泰国北部、泰国半岛和苏梅岛种群。
