Department of Endocrinology, the First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Biopolymers. 2010 Jul;93(7):587-94. doi: 10.1002/bip.21389.
Glucose acts as a beta-cell stimulus factor and leads to cellular responses that involve a large amount of biomolecule formation, relocation, and transformation. We hypothesize that information about these changes can be obtained in real-time by laser tweezers Raman spectroscopy. To test this hypothesis, repeated measurements designs in accordance with the application of Raman spectroscopy detection were used in the current experiment. Single rat beta-cells were measured by Raman spectroscopy in 2.8 mmol/l glucose culture medium as a basal condition. After stimulation with high glucose (20 mmol/l), the same cells were measured continuously. Each cell was monitored over a total time span of 25 min, in 5 min intervals. During this period of time, cells were maintained at an appropriate temperature controlled by an automatic heater, to provide near-physiological conditions. It was found that some significant spectral changes induced by glucose were taking place during the stimulation time course. The most noticeable changes were the increase of spectral intensity at the 1002, 1085, 1445, and 1655 cm(-1) peaks, mainly corresponding to protein and lipid. We speculate that these changes might have to do with beta-cell protein and lipid synthesis. Using laser tweezers Raman spectroscopy in combination with glucose stimulation, optical spectral information from rat beta-cells was received and analyzed.
葡萄糖作为β细胞刺激因子，导致细胞产生大量生物分子的形成、重定位和转化等反应。我们假设，这些变化的信息可以通过激光镊子拉曼光谱实时获得。为了验证这一假设，本实验采用符合拉曼光谱检测应用的重复测量设计。在 2.8mmol/L 葡萄糖培养基中，用拉曼光谱测量单个大鼠β细胞作为基础条件。用高葡萄糖（20mmol/L）刺激后，连续测量相同的细胞。每个细胞在 25 分钟的总时间跨度内以 5 分钟的间隔进行监测。在此期间，细胞在自动加热器控制下保持在适当的温度下，以提供接近生理的条件。结果发现，在刺激过程中发生了一些由葡萄糖诱导的显著光谱变化。最明显的变化是在 1002、1085、1445 和 1655cm(-1) 峰处的光谱强度增加，主要对应于蛋白质和脂质。我们推测这些变化可能与β细胞蛋白质和脂质的合成有关。使用激光镊子拉曼光谱结合葡萄糖刺激，从大鼠β细胞接收和分析光学光谱信息。
