Department of Environmental Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
Bioresour Technol. 2010 May;101(10):3394-9. doi: 10.1016/j.biortech.2009.12.047. Epub 2010 Jan 25.
The influence of metal ions, anions, ortho-phosphate, polyphosphate and organic phosphorus on the activity and kinetics of alkaline phosphatase in aerobic activated sludge taken from a local municipal wastewater treatment plant was investigated. Results suggested that there were three impact models of heavy metals on alkaline phosphatase activity (APA) and most inorganic anions were relatively innocuous at concentrations from 0 to 5.0mM. However, phosphorus especially pyrophosphate had an inhibitory effect on APA, since the residual activity of alkaline phosphatase dramatically decreased to 34.8% of the control at the pyrophosphate concentration of 5.0mM. The inhibition of Cu(2+) on alkaline phosphatase was described satisfactorily by an uncompetitive type at low concentrations, but a mixed of noncompetitive-uncompetitive type at high concentrations. The inhibition of ortho-phosphate was found to be of the competitive type. V(max)/K(m) ratio is a good indicator for the catalytic efficiency of alkaline phosphatases in wastewater.
研究了来自当地城市污水处理厂的需氧活性污泥中金属离子、阴离子、正磷酸盐、多聚磷酸盐和有机磷对碱性磷酸酶活性和动力学的影响。结果表明，重金属对碱性磷酸酶活性（APA）有三种影响模式，大多数无机阴离子在 0 到 5.0mM 的浓度下相对无害。然而，磷特别是焦磷酸盐对 APA 具有抑制作用，因为在 5.0mM 焦磷酸盐浓度下，碱性磷酸酶的剩余活性急剧下降到对照的 34.8%。Cu(2+) 对碱性磷酸酶的抑制作用在低浓度下可以很好地用非竞争性类型来描述，但在高浓度下是混合的非竞争性-非竞争性类型。正磷酸盐的抑制作用被发现是竞争性的。V(max)/K(m) 比值是废水碱性磷酸酶催化效率的一个很好的指标。
