Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 You Yi Road, Chongqing 400016, China.
Seizure. 2010 Mar;19(2):79-83. doi: 10.1016/j.seizure.2009.12.001. Epub 2010 Jan 25.
In epilepsy, many studies were focus on apoE gene polymorphism and found APOE epsilon4 to be associated with earlier onset of temporal lobe epilepsy. There is not any study about apoE in cerebrospinal fluid (CSF) of epileptic patients, so we detect the CSF-apoE and determine whether it is changed after seizure.
A total of 60 epileptic patients and 28 subjects with no evidence of any neurological diseases were studied. The concentrations of CSF-apoE were detected with enzyme-linked immunosorbent assay.
The CSF-apoE levels in epilepsy and control group were 5.78+/-2.15 mg/l and 13.60+/-12.11 mg/l, and there were statistical difference. In epilepsy group, the CSF-apoE concentration was 6.53+/-2.55 mg/l in male patients, and 4.98+/-1.21 mg/l in female. In secondary epilepsy group was 5.06+/-1.31 mg/l, and in idiopathic epilepsy was 6.04+/-2.34 mg/l. In different seizure types groups, including complex partial seizure (CPS), secondarily generalized tonic-clonic seizure (SGTC), generalized tonic-clonic seizure (GTCS), and absence seizure (AS), the mean concentrations of CSF-apoE were 6.62+/-3.13 mg/l, 5.21+/-1.22 mg/l, 5.00+/-1.09 mg/l and 7.25+/-1.88 mg/l, respectively.
CSF-apoE concentration decreases after seizures, correlated with the gender, etiological factor and seizure types.
在癫痫中，许多研究集中在 apoE 基因多态性上，发现 APOE epsilon4 与颞叶癫痫的发病年龄较早有关。目前还没有关于癫痫患者脑脊液（CSF）中 apoE 的研究，因此我们检测了 CSF-apoE，并确定其在癫痫发作后是否发生变化。
共纳入 60 例癫痫患者和 28 例无任何神经系统疾病证据的对照者。采用酶联免疫吸附试验检测 CSF-apoE 浓度。
癫痫组和对照组的 CSF-apoE 水平分别为 5.78+/-2.15mg/l 和 13.60+/-12.11mg/l，差异有统计学意义。在癫痫组中，男性患者的 CSF-apoE 浓度为 6.53+/-2.55mg/l，女性患者为 4.98+/-1.21mg/l。继发性癫痫组为 5.06+/-1.31mg/l，特发性癫痫组为 6.04+/-2.34mg/l。在不同发作类型组中，包括复杂部分性发作（CPS）、继发性全面强直阵挛发作（SGTC）、全面强直阵挛发作（GTCS）和失神发作（AS），CSF-apoE 的平均浓度分别为 6.62+/-3.13mg/l、5.21+/-1.22mg/l、5.00+/-1.09mg/l 和 7.25+/-1.88mg/l。
癫痫发作后 CSF-apoE 浓度降低，与性别、病因和发作类型有关。
