Rubinchik Evelina, Pasetka Christopher
MIGENIX Inc., Vancouver, BC, Canada.
Methods Mol Biol. 2010;618:349-58. doi: 10.1007/978-1-60761-594-1_21.
It is widely accepted that cationic antimicrobial peptides possess potent microbicidal properties. Recent studies show that in addition to their antimicrobial action, these peptides can exhibit anti-inflammatory activity. The purpose of this chapter is to describe in vivo ear inflammation models that can be used for evaluating the anti-inflammatory activity of antimicrobial peptides. The models are based on different mechanisms of inflammation development and include irritant dermatitis (a model induced by a single application of 12-o-tetradecanoylphorbol acetate [TPA]) and allergic dermatitis, or delayed type hypersensitivity reaction (a model induced by repetitive application of oxazolone).
阳离子抗菌肽具有强大的杀菌特性，这一点已被广泛接受。最近的研究表明，除了抗菌作用外，这些肽还可表现出抗炎活性。本章的目的是描述可用于评估抗菌肽抗炎活性的体内耳部炎症模型。这些模型基于不同的炎症发展机制，包括刺激性皮炎（由单次应用12 - O - 十四酰佛波醇 - 13 - 乙酸酯[TPA]诱导的模型）和过敏性皮炎，即迟发型超敏反应（由反复应用恶唑酮诱导的模型）。
