Department of Physiology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil.
Phytomedicine. 2010 Aug;17(10):740-3. doi: 10.1016/j.phymed.2010.01.006. Epub 2010 Feb 9.
The anti-inflammatory effect of physalin E, a seco-steroid isolated from Physalis angulata L. was evaluated on acute and chronic models of dermatitis induced by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and oxazolone, respectively, in mouse ear. The changes in ear edema/thickness, production of pro-inflammatory cytokines (TNF-alpha and IFN-gamma), myeloperoxidase (MPO) activity, and histological and immunohistochemical findings were analysed, as indicators of dermal inflammation. Similar to dexamethasone, topically applied Physalin E (0.125; 0.25 and 0.5 mg/ear) potently inhibited the TPA and oxazolone-induced dermatitis, leading to substantial reductions in ear edema/thickness, pro-inflammatory cytokines, and MPO activity. These effects were reversed by mifepristone, a steroid antagonist and confirmed by immunohistochemical and histopathological analysis. The data suggest that physalin E may be a potent and topically effective anti-inflammatory agent useful to treat the acute and chronic skin inflammatory conditions.
从黄灯笼（Physalis angulata L.）中分离得到的甾体化合物 physalin E 具有抗炎作用，分别在 TPA 和 oxazolone 诱导的急性和慢性皮炎模型中进行了评估。以耳肿胀/厚度、促炎细胞因子（TNF-α和 IFN-γ）、髓过氧化物酶（MPO）活性以及组织学和免疫组织化学发现为指标，分析了皮肤炎症的变化。与地塞米松类似，局部应用 physalin E（0.125；0.25 和 0.5 mg/耳）可有效抑制 TPA 和 oxazolone 诱导的皮炎，导致耳肿胀/厚度、促炎细胞因子和 MPO 活性显著降低。这些作用被甾体拮抗剂米非司酮逆转，并通过免疫组织化学和组织病理学分析得到证实。数据表明，physalin E 可能是一种有效的局部抗炎药物，可用于治疗急性和慢性皮肤炎症。
