Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Luminescence. 2011 Mar-Apr;26(2):106-11. doi: 10.1002/bio.1191.
A new spectrofluorimetric method was reported for the determination of folic acid (FA), based on its quenching effect on the fluorescence intensity of Tb(3+) -1,10-phenanthroline complex as a fluorescent probe. The quenched fluorescence intensity at an emission wavelength of 545 nm was proportional to the concentration of FA in Tris-HCl buffer solution of pH 6.2. The effects of pH, time, order of addition of reagents, temperature and concentrations of Tb(3+) , buffer and 1,10-phenanthroline were investigated and optimized. The linear range for the determination of FA was 0.01-1.1 mg/L. The detection limit was 0.003 mg/L and the relative standard deviation for replicated determination of 1 mg/L of folic acid was 1.2%. This method was simple, practical and relatively free from interference effects. It was successfully applied to assess FA in pharmaceutical tablets and urine samples.
一种新的荧光分光光度法被报道用于测定叶酸（FA），其基础是叶酸对作为荧光探针的铽（Tb（3+））-1,10-菲啰啉络合物荧光强度的猝灭效应。在发射波长为 545nm 时，猝灭荧光强度与 pH 值为 6.2 的 Tris-HCl 缓冲溶液中 FA 的浓度成正比。考察并优化了 pH 值、时间、试剂加入顺序、温度以及 Tb（3+）、缓冲液和 1,10-菲啰啉浓度的影响。FA 的测定线性范围为 0.01-1.1mg/L。检测限为 0.003mg/L，1mg/L 叶酸的重复性测定的相对标准偏差为 1.2%。该方法简单、实用，相对不受干扰影响。它成功地应用于评估药物片剂和尿液样品中的 FA。
