Medical Chemistry Staff Room, Weifang Medical University, Weifang, Shandong, People's Republic of China.
Luminescence. 2011 Jan-Feb;26(1):54-8. doi: 10.1002/bio.1185. Epub 2010 Jan 21.
A new spectrofluorimetric method was developed for determination of trace amount of bilirubin. Using oxytetracycline-Eu(3+) as a fluorescent probe, in the buffer solution of pH = 7.3, bilirubin can reduce remarkably the fluorescence intensity of the oxytetracycline-Eu(3+) complex at λ = 612 nm and the reduced fluorescence intensity was in proportion to the concentration of bilirubin. Optimum conditions for the determination of bilirubin were also investigated. The linear range and limit of detection for the determination of bilirubin were 5.0 × 10(-7) , 3.0 × 10(-5) and 7.7 × 10(-8) mol L(-1) , respectively. This method is simple, practical and relatively free of interference from coexisting substances and can be successfully applied to assess bilirubin in serum samples and compared with the modified Jendrassik-Grof method in clinical analysis. The quenching mechanism of the fluorescence intensity in the system is also discussed.
建立了一种测定痕量胆红素的新的荧光光度法。在 pH = 7.3 的缓冲溶液中，以土霉素-Eu(3+)为荧光探针，胆红素能显著降低土霉素-Eu(3+)络合物在 λ = 612nm 处的荧光强度，且其荧光强度的降低与胆红素的浓度成正比。还对测定胆红素的最佳条件进行了探讨。该方法测定胆红素的线性范围和检出限分别为 5.0×10(-7)、3.0×10(-5)和 7.7×10(-8)mol·L(-1)。该法简便、实用，且共存物质的干扰较小，可成功地用于血清样品中胆红素的测定，并与改良 Jendrassik-Grof 法进行了临床分析比较。还讨论了该体系中荧光强度猝灭的机理。
