新型超级燕麦品种（CDC SO-I 与传统品种相比）的结构组成、生物聚合物构象和生物降解特性：一种新方法。

Structural makeup, biopolymer conformation, and biodegradation characteristics of a newly developed super genotype of oats (CDC SO-I versus conventional varieties): a novel approach.

机构信息

Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 5A8.

出版信息

J Agric Food Chem. 2010 Feb 24;58(4):2377-87. doi: 10.1021/jf903514t.

DOI:10.1021/jf903514t

PMID:20095614

Abstract

Recently, a new "super" genotype of oats (CDC SO-I or SO-I) has been developed. The objectives of this study were to determine structural makeup (features) of oat grain in endosperm and pericarp regions and to reveal and identify differences in protein amide I and II and carbohydrate structural makeup (conformation) between SO-I and two conventional oats (CDC Dancer and Derby) grown in western Canada in 2006, using advanced synchrotron radiation based Fourier transform infrared microspectroscopy (SRFTIRM). The SRFTIRM experiments were conducted at National Synchrotron Light Sources, Brookhaven National Laboratory (NSLS, BNL, U.S. Department of Energy). From the results, it was observed that comparison between the new genotype oats and conventional oats showed (1) differences in basic chemical and protein subfraction profiles and energy values with the new SO-I oats containing lower lignin (21 g/kg of DM) and higher soluble crude protein (530 g/kg CP), crude fat (59 g/kg of DM), and energy values (TDN, 820 g/kg of DM; NE(L3x), 7.8 MJ/kg of DM); (2) significant differences in rumen biodegradation kinetics of dry matter, starch, and protein with the new SO-I oats containing lower EDDM (638 g/kg of DM) and higher EDCP (103 g/kg of DM); (3) significant differences in nutrient supply with highest truly absorbed rumen undegraded protein (ARUP, 23 g/kg of DM) and total metabolizable protein supply (MP, 81 g/kg of DM) from the new SO-I oats; and (4) significant differences in structural makeup in terms of protein amide I in the endosperm region (with amide I peak height from 0.13 to 0.22 IR absorbance unit) and cellulosic compounds to carbohydrate ratio in the pericarp region (ratio from 0.02 to 0.06). The results suggest that with the SRFTIRM technique, the structural makeup differences between the new genotype oats (SO-I) and two conventional oats (Dancer and Derby) could be revealed.

摘要

最近，一种新的“超级”燕麦品种（CDC SO-I 或 SO-I）已经开发出来。本研究的目的是确定胚乳和果皮区域中燕麦籽粒的结构组成（特征），并利用先进的基于同步加速器辐射的傅里叶变换红外显微镜（SRFTIRM）揭示和识别 2006 年在加拿大西部种植的 SO-I 与两种传统燕麦（CDC Dancer 和 Derby）之间在酰胺 I 和 II 蛋白和碳水化合物结构组成（构象）方面的差异。SRFTIRM 实验在美国能源部布鲁克海文国家实验室的国家同步加速器光源（NSLS，BNL）进行。结果表明，与传统燕麦相比，新基因型燕麦的比较结果显示：（1）基本化学成分和蛋白质亚组分谱以及能量值存在差异，新的 SO-I 燕麦含有较低的木质素（21 g/kg DM）和较高的可溶性粗蛋白（530 g/kg CP）、粗脂肪（59 g/kg DM）和能量值（TDN，820 g/kg DM；NE（L3x），7.8 MJ/kg DM）；（2）干物质、淀粉和蛋白质的瘤胃生物降解动力学存在显著差异，新的 SO-I 燕麦含有较低的 EDDM（638 g/kg DM）和较高的 EDCP（103 g/kg DM）；（3）新的 SO-I 燕麦具有最高的真正可吸收瘤胃未降解蛋白（ARUP，23 g/kg DM）和总可代谢蛋白供应（MP，81 g/kg DM），提供的养分供应存在显著差异；（4）在胚乳区域的酰胺 I 结构组成（酰胺 I 峰高为 0.13 至 0.22 IR 吸光度单位）和果皮区域的纤维素化合物与碳水化合物的比例（比率为 0.02 至 0.06）方面存在显著差异。结果表明，利用 SRFTIRM 技术，可以揭示新基因型燕麦（SO-I）与两种传统燕麦（Dancer 和 Derby）之间的结构组成差异。