Tu Jiayu, Chen Qingyun, Zhou Junyan, Fan Yuxin, Li Yanlong, Ma Yonghang, Zeng Xiangfang, Qiao Shiyan, Cai Shuang
State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture and Rural Affairs Feed Industry Centre, China Agricultural University, Beijing 100193, China.
Frontier Technology Research Institute of China Agricultural University in Shenzhen, Shenzhen 518116, China.
Animals (Basel). 2025 May 22;15(11):1510. doi: 10.3390/ani15111510.
The objective of this study is to investigate the digestion and metabolism characteristics of amino acids and glucose in energy and protein feeds, and to establish regression equations to accurately predict their release rates in vivo based on the in vitro digestion characteristics of the feedstuffs. A total of 9 energy feedstuffs and 19 protein feedstuffs were selected for in vitro digestion simulation experiments. Additionally, four representative energy and protein feeds were then chosen for the portal vein, femoral artery, and femoral vein blood cannulation experiments in growing pigs. The results showed that among the nine energy feedstuffs tested, wheat bran presented the highest degree of digestion, followed by wheat, whereas potato had the lowest degree of digestion. The digestibility of starch at different time points in vitro was linearly correlated with the crude fiber content, total starch content, and ratio of amylose to amylopectin (R = 0.610.96). Among the 19 protein feedstuffs tested, peas released the highest total amount of amino acids, followed by sugar beet meal. The in vivo digestibility and metabolism trial in cannulated pigs showed that the total amino acid release was linearly correlated with the in vitro amino acid release rates, dry matter, crude protein, neutral detergent fiber, crude fat, and total energy of the feedstuffs (R = 0.930.99).
本研究的目的是探究能量和蛋白质饲料中氨基酸和葡萄糖的消化代谢特性,并基于饲料原料的体外消化特性建立回归方程,以准确预测它们在体内的释放率。总共选择了9种能量饲料和19种蛋白质饲料进行体外消化模拟实验。此外,随后选择了四种具有代表性的能量和蛋白质饲料,对生长猪进行门静脉、股动脉和股静脉插管实验。结果表明,在所测试的9种能量饲料中,麦麸的消化程度最高,其次是小麦,而马铃薯的消化程度最低。体外不同时间点淀粉的消化率与粗纤维含量、总淀粉含量以及直链淀粉与支链淀粉的比例呈线性相关(R = 0.610.96)。在所测试的19种蛋白质饲料中,豌豆释放的氨基酸总量最高,其次是甜菜粕。对插管猪进行的体内消化率和代谢试验表明,总氨基酸释放量与饲料原料的体外氨基酸释放率、干物质、粗蛋白、中性洗涤纤维、粗脂肪和总能呈线性相关(R = 0.930.99)。