Savela K, Hemminki K
Institute of Occupational Health, Helsinki, Finland.
Carcinogenesis. 1991 Mar;12(3):503-8. doi: 10.1093/carcin/12.3.503.
The effects of smoking and DNA adduct formation were analysed in isolated human white blood cell populations. As the white cells are composed mainly of granulocytes with a short half-life and T-lymphocytes with a half-life of several years, we isolated the lymphocytes and granulocytes of 11 smokers and 10 nonsmokers to determine any smoking-related DNA adducts by the nuclease-P1-enhanced 32P-postlabelling assay. The differences between the mean lymphocyte DNA adducts/10(8) nucleotides of 31 +/- 5.7 (SE) of smokers were significantly higher (P less than 0.05) than those in the lymphocytes 13 +/- 1.6 (SE) of nonsmokers. The total DNA adducts/10(8) nucleotides obtained from the granulocytes of smokers and nonsmokers was 9.6 +/- 1.9 and 7.6 +/- 1.9 respectively. The plasma cotinine concentrations were in good agreement with the smoking information given by the individual smokers (r = 0.847, P less than 0.001). The DNA adduct levels of the lymphocytes of the 10 smokers correlated with the plasma cotinine concentrations (r = 0.639, P less than 0.05). The variation between the results was explained by the variation among the individuals and the samples, but not by the variation in the parallel determinations. More detailed studies are needed to analyse the source of the individual variations between the smokers' adduct levels, DNA repair, and differences in the metabolism of the compounds in cigarette smoke.
在分离出的人体白细胞群体中分析了吸烟及DNA加合物形成的影响。由于白细胞主要由半衰期较短的粒细胞和半衰期为数年的T淋巴细胞组成，我们分离了11名吸烟者和10名不吸烟者的淋巴细胞和粒细胞，通过核酸酶P1增强的32P后标记分析法来确定任何与吸烟相关的DNA加合物。吸烟者平均淋巴细胞DNA加合物/10(8)核苷酸为31±5.7（标准误），显著高于不吸烟者淋巴细胞中的13±1.6（标准误）（P<0.05）。吸烟者和不吸烟者粒细胞中获得的总DNA加合物/10(8)核苷酸分别为9.6±1.9和7.6±1.9。血浆可替宁浓度与个体吸烟者提供的吸烟信息高度一致（r = 0.847，P<0.001）。10名吸烟者淋巴细胞的DNA加合物水平与血浆可替宁浓度相关（r = 0.639，P<0.05）。结果之间的差异可由个体和样本间的差异解释，但不能由平行测定中的差异解释。需要进行更详细的研究来分析吸烟者加合物水平个体差异的来源、DNA修复以及香烟烟雾中化合物代谢的差异。
