Department of Dermatology of University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstrasse1, 55101 Mainz, Germany.
J Immunol Methods. 2010 Feb 28;353(1-2):62-70. doi: 10.1016/j.jim.2010.01.002. Epub 2010 Jan 22.
Natural CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers and to identify targets for a therapeutic modulation of Tregs. Here we present a methodology optimized to efficiently induce and select mAb against human Tregs by repeated immunization of mice with Tregs from a single donor and a differential two-step flow cytometry-based hybridoma screening procedure.
天然 CD4(+)CD25(+)Foxp3(+) 调节性 T 细胞 (Tregs) 控制着免疫系统的激活，因此成为免疫学研究的一个主要领域。针对人类 Tregs 生成单克隆抗体为发现新的 Treg 特异性或 Treg 相关表面标志物以及鉴定 Treg 治疗调节的靶标提供了可能。在此，我们提出了一种经优化的方法，该方法通过用来自单个供体的 Tregs 对小鼠进行重复免疫，并采用基于两步流式细胞术的差异杂交瘤筛选程序，可有效诱导和选择针对人 Tregs 的 mAb。
