Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 47416-95447, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Apr;75(4):1270-4. doi: 10.1016/j.saa.2009.12.048. Epub 2009 Dec 22.
Resolution of binary mixtures of paracetamol, phenylephrine hydrochloride and chlorpheniramine maleate with minimum sample pre-treatment and without analyte separation has been successfully achieved by methods of partial least squares algorithm with one dependent variable, principal component regression and hybrid linear analysis. Data of analysis were obtained from UV-vis spectra of the above compounds. The method of central composite design was used in the ranges of 1-15 mg L(-1) for both calibration and validation sets. The models refinement procedure and their validation were performed by cross-validation. Figures of merit such as selectivity, sensitivity, analytical sensitivity and limit of detection were determined for all three compounds. The procedure was successfully applied to simultaneous determination of the above compounds in pharmaceutical tablets.
通过偏最小二乘算法、主成分回归和混合线性分析等方法，无需对样品进行预处理且无需分离分析物，成功地对扑热息痛、盐酸苯肾上腺素和马来酸氯苯那敏的二元混合物进行了定量分析。分析数据来自于上述化合物的紫外-可见光谱。在 1-15 mg L(-1) 的范围内，采用中心复合设计方法对校准集和验证集进行分析。通过交叉验证对模型的精炼过程及其验证进行了处理。对所有三种化合物都测定了选择性、灵敏度、分析灵敏度和检测限等质量参数。该程序成功地应用于同时测定药物片中的上述化合物。
