Del Re Brunella, Marcantonio Pamela, Bersani Ferdinando, Giorgi Gianfranco
Department of Evolutionary Experimental Biology, University of Bologna, via Selmi 3, 40126 Bologna, Italy.
Gen Physiol Biophys. 2009 Dec;28(4):420-4. doi: 10.4149/gpb_2009_04_420.
In our earlier experiments, we found that extremely low frequency magnetic fields (ELF-MF) affect heat shock protein (HSP) expression in wild type Escherichia coli cells. In the present work we investigate the ability of ELF-MF exposure to trigger an increase of DnaK and GroEL protein levels also in E. coli cells not exhibiting the classic heat shock response (HSR) when subjected to a 42 degrees C heat stress. We find that these cells, although lacking a HSR to heat shock treatment, show an enhancement of DnaK and GroEL protein levels after 30 or 90 min sinusoidal ELF-MF exposure (50 Hz, 1 mT). This result suggests that the HSP induction pathway triggered by ELF-MF exposure could be different from that elicited by heat shock treatment.
在我们早期的实验中，我们发现极低频磁场（ELF-MF）会影响野生型大肠杆菌细胞中热休克蛋白（HSP）的表达。在本研究中，我们探究了暴露于ELF-MF是否也能促使未表现出经典热休克反应（HSR）的大肠杆菌细胞在经受42摄氏度热应激时，其DnaK和GroEL蛋白水平增加。我们发现，这些细胞虽然对热休克处理缺乏HSR，但在暴露于正弦ELF-MF（50赫兹，1毫特斯拉）30或90分钟后，DnaK和GroEL蛋白水平有所增强。这一结果表明，ELF-MF暴露引发的HSP诱导途径可能不同于热休克处理引发的途径。
