Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
Nanotechnology. 2010 Feb 26;21(8):85501. doi: 10.1088/0957-4484/21/8/085501. Epub 2010 Jan 25.
A novel and simple method for gold nanoshell synthesis with controllable core and shell sizes is reported here. A new 'tree-shape' surfactant bis(amidoethyl-carbamoylethyl) octadecylamine (C18N3) was synthesized and used as the glue for the fast combination of gold nanoparticles and the subsequent gold shell outside. The functionalized polystyrene (PS) cores were covered by a surfactant (PS@C18N3) bilayer. The presence of the surfactant double layer played the role of 'glue' in this method, so that upon controlling the amount of surfactant, it was possible to achieve: the manipulation of gold seed density on the PS@C18N3 spheres, the preparation of PS@Au hybrid structures, and a red-shift in the extinction absorption from 520 to 750 nm. Besides, the as-prepared PS@Au composites supported on a glass substrate exhibited excellent effectiveness in the molecular recognition of human-immunoglobulin G (h-IgG) and goat anti-human-immunoglobulin G (goat anti-h-IgG), showing a rapid response within 20 min with a low detection limit of 10 ng ml(-1). This demonstrates that PS@Au prepared and assembled using our method is potentially useful as a nanosensor platform for immunoassay.
本文报道了一种新颖且简单的方法，用于合成具有可控核壳尺寸的金纳米壳。合成了一种新型的“树形”表面活性剂双（酰胺乙基-氨甲酰乙基）十八烷基胺（C18N3），并将其用作快速结合金纳米粒子和随后的金壳外的胶。功能化聚苯乙烯（PS）核被表面活性剂（PS@C18N3）双层覆盖。表面活性剂双层的存在在这种方法中起到了“胶”的作用，因此通过控制表面活性剂的量，可以实现：在 PS@C18N3 球上操纵金种子的密度，制备 PS@Au 杂化结构，以及将消光谱从 520nm 红移到 750nm。此外，在玻璃基底上支撑的制备的 PS@Au 复合材料在人免疫球蛋白 G（h-IgG）和山羊抗人免疫球蛋白 G（goat anti-h-IgG）的分子识别中表现出优异的效果，在 20 分钟内快速响应，检测限低至 10ng ml(-1)。这表明使用我们的方法制备和组装的 PS@Au 作为免疫分析的纳米传感器平台具有潜在的用途。
