The New Zealand Institute for Plant and Food Research Limited, P.O. Box 4704, Christchurch, New Zealand.
Plant Cell Rep. 2010 Mar;29(3):223-30. doi: 10.1007/s00299-009-0814-z. Epub 2010 Jan 23.
Transgenic garlic (Allium sativum) plants have been recovered directly from immature leaf material by selective culture following Agrobacterium-mediated transformation. This method involved the use of a binary vector containing the mgfp-ER reporter gene and hpt selectable marker, and followed a similar protocol developed previously for the transformation of immature onion embryos. The choice of tissue and post-transformation selection procedure resulted in a large increase in recovery of transgenic plants compared with previously confirmed allium transformation protocols. The presence of transgenes in the genome of the plants was confirmed using Southern analysis. This improvement in frequency and the use of clonal commercial "Printanor" germplasm now makes possible the integration of useful agronomic and quality traits into this crop.
通过农杆菌介导的转化，从成熟叶组织中直接获得了转基因大蒜（Allium sativum）植株。该方法使用了含有 mgfp-ER 报告基因和 hpt 选择标记的二元载体，并遵循了先前为不成熟洋葱胚胎转化开发的类似方案。与先前确认的所有转化方案相比，组织选择和转化后选择程序的选择导致转基因植物的回收率大大增加。使用 Southern 分析证实了这些植物基因组中存在转基因。这种频率的提高以及对商业“Printanor”种质资源的克隆利用，使得将有用的农艺和品质性状整合到这种作物中成为可能。
