Nishihara Masahiro, Nakatsuka Takashi
Iwate Biotechnology Research Center, Narita, Kitakami, Iwate, Japan.
Methods Mol Biol. 2010;589:325-47. doi: 10.1007/978-1-60327-114-1_29.
Since the first successful genetic engineering of flower color in petunia, several new techniques have been developed and applied to modify flower color not only in model plants but also in floricultural plants. A typical example is the commercial violet-flowered carnation "Moondust series" developed by Suntry Ltd. and Florigene Ltd. More recently, blue-flowered roses have been successfully produced and are expected to be commercially available in the near future. In recent years, successful modification of flower color by sophisticated regulation of flower-pigment metabolic pathways has become possible. In this chapter, we review recent advances in flower color modification by genetic engineering, especially focusing on the methodology. We have included our own recent results on successful production of flower-color-modified transgenic plants in a model plant, tobacco and an ornamental plant, gentian. Based on these results, genetic engineering of flower color for improvement of floricultural plants is discussed.
自从矮牵牛首次成功进行花色的基因工程改造以来，已经开发出了几种新技术，并应用于不仅在模式植物而且在花卉植物中改变花色。一个典型的例子是由三得利有限公司和Florigene有限公司培育的商业化紫花康乃馨“月光系列”。最近，蓝色玫瑰已成功培育出来，预计在不久的将来可投入商业生产。近年来，通过对花色素代谢途径进行精细调控成功改变花色已成为可能。在本章中，我们综述了通过基因工程改变花色的最新进展，尤其侧重于方法学。我们纳入了我们自己最近在模式植物烟草和观赏植物龙胆中成功培育出花色改良转基因植物的结果。基于这些结果，讨论了用于改良花卉植物的花色基因工程。
