College of Horticulture, Key Laboratory of Protected Horticulture (Ministry of Education), College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan.
BMC Plant Biol. 2020 Sep 29;20(1):446. doi: 10.1186/s12870-020-02658-z.
Petals are the colorful region of many ornamental plants. Quality traits of petal color directly affect the value of ornamental plants. Although the regulatory mechanism of flower color has been widely studied in many plants, that of lily flower color is still worth further exploration.
In this study, the pigmentation regulatory network in different regions of the petal of lily cultivar 'Vivian' was analyzed through tissue structure, metabolites biosynthesis, and gene expression. We found that cell morphology of the petal in un-pigmented region differed from that in pigmented region. The cell morphology tends to flatten in un-pigmented region where the color is lighter. Moreover, high level anthocyanin was found in the pigmented regions by metabonomic analysis, especially cyanidin derivatives. However, flavanones were accumulated, contrast with anthocyanin in the un-pigmented regions of lily petal. To understand the relationship of these different metabolites and lily flower color, RNA-Seq was used to analyze the differentially expressed genes-related metabolite biosynthesis. Among these genes, the expression levels of several genes-related cyanidin derivatives biosynthesis were significantly different between the pigmented and un-pigmented regions, such as LvMYB5, LvMYB7, LvF3'H, LvDFR, LvANS and Lv3GT.
This data will help us to further understand the regulation network of lily petal pigmentation and create different unique color species.
花瓣是许多观赏植物色彩斑斓的区域。花瓣颜色的质量特征直接影响观赏植物的价值。尽管许多植物的花色素调控机制已得到广泛研究，但百合花色的调控机制仍值得进一步探索。
本研究通过组织学结构、代谢物生物合成和基因表达分析了百合品种‘Vivian’花瓣不同区域的色素调控网络。我们发现，未着色区域的花瓣细胞形态与着色区域的不同。未着色区域的颜色较浅，细胞形态趋于扁平。此外，代谢组学分析发现着色区域含有高水平的花青素，特别是飞燕草素衍生物。然而，在百合花瓣的未着色区域积累了类黄酮，与花青素形成对比。为了了解这些不同代谢物与百合花色的关系，我们使用 RNA-Seq 分析了与代谢物生物合成相关的差异表达基因。在这些基因中，几个与飞燕草素衍生物生物合成相关的基因的表达水平在着色和非着色区域之间有显著差异，如 LvMYB5、LvMYB7、LvF3'H、LvDFR、LvANS 和 Lv3GT。
这些数据将有助于我们进一步了解百合花瓣色素形成的调控网络，并创造出不同独特颜色的品种。
