Breyton Cécile, Pucci Bernard, Popot Jean-Luc
Institut de Biologie Structurale, UMR CNRS/CEA/UJF, Grenoble, France.
Methods Mol Biol. 2010;601:219-45. doi: 10.1007/978-1-60761-344-2_14.
Handling integral membrane proteins in aqueous solutions traditionally relies on the use of detergents, which are surfactants capable of dispersing the components of biological membranes into mixed micelles. The dissociating character of detergents, however, most often causes solubilized membrane proteins to be unstable. This has prompted the development of alternative, less-aggressive surfactants designed to keep membrane proteins soluble, after they have been solubilized, under milder conditions. A short overview is presented of the structure, properties, and uses of two families of such surfactants: amphiphilic polymers ("amphipols") and fluorinated surfactants.
传统上，在水溶液中处理整合膜蛋白依赖于使用去污剂，去污剂是一类能够将生物膜的成分分散到混合胶束中的表面活性剂。然而，去污剂的解离特性常常导致溶解的膜蛋白不稳定。这促使人们开发替代性的、温和性较低的表面活性剂，旨在使膜蛋白在温和条件下溶解后仍保持可溶状态。本文简要概述了两类此类表面活性剂的结构、性质和用途：两亲聚合物（“双亲分子”）和氟化表面活性剂。
