Meister Annette, Blume Alfred
Institute of Chemistry, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
Polymers (Basel). 2017 Oct 19;9(10):521. doi: 10.3390/polym9100521.
Lipid membranes can incorporate amphiphilic or polyphilic molecules leading to specific functionalities and to adaptable properties of the lipid bilayer host. The insertion of guest molecules into membranes frequently induces changes in the shape of the lipid matrix that can be visualized by transmission electron microscopy (TEM) techniques. Here, we review the use of stained and vitrified specimens in (cryo)TEM to characterize the morphology of amphiphilic and polyphilic molecules upon insertion into phospholipid model membranes. Special emphasis is placed on the impact of novel synthetic amphiphilic and polyphilic bolalipids and polymers on membrane integrity and shape stability.
脂质膜可以包含两亲性或多亲性分子，从而赋予脂质双层主体特定的功能和可适应的特性。客体分子插入膜中常常会引起脂质基质形状的变化，这种变化可以通过透射电子显微镜（TEM）技术观察到。在这里，我们综述了在（低温）TEM中使用染色和玻璃化标本，以表征两亲性和多亲性分子插入磷脂模型膜后的形态。特别强调了新型合成两亲性和多亲性bola脂质和聚合物对膜完整性和形状稳定性的影响。
