在与TFIID的竞争中，NFI对腺病毒末端反向转录的抑制作用。

The repression of the reverse-oriented transcription from the adenovirus terminus by NFI in competition with TFIID.

作者信息

Matsumoto K, Nagata K, Hanaoka F, Ui M

机构信息

Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.

出版信息

FEBS Lett. 1991 Mar 11;280(1):53-6. doi: 10.1016/0014-5793(91)80202-e.

DOI:10.1016/0014-5793(91)80202-e

PMID:2009968

Abstract

Nuclear factor I (NF) represses the transcription which is promoted by the cloned adenovirus (Ad) type 5 DNA replication origin and is reverse-oriented with respect to the direction of the replication. The mechanism of this repression by NFI was investigated. In the cell-free transcription system, the repression was observed only when NFI was present during the formation of the transcription initiation complex. From the results of DNase I protection experiments, it was indicated that NFI bound to its binding site in the Ad replication origin prevents TFIID from proper binding to the adjacent AT-rich region and consequently represses the transcription.

摘要

核因子I（NF）抑制由克隆的5型腺病毒（Ad）DNA复制起点所促进且相对于复制方向呈反向的转录。研究了NFI这种抑制作用的机制。在无细胞转录系统中，仅当在转录起始复合物形成过程中存在NFI时才观察到这种抑制作用。从脱氧核糖核酸酶I保护实验结果表明，NFI与其在腺病毒复制起点中的结合位点结合会阻止TATA盒结合蛋白（TFIID）与相邻富含AT的区域正常结合，从而抑制转录。