Departamento de Quimica Organica-CIHIDECAR, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, C1428 Buenos Aires, Argentina.
J Agric Food Chem. 2010 Feb 24;58(4):2562-70. doi: 10.1021/jf9039099.
Climacteric Japanese plums were harvested at six developmental stages with no intermediate storage period, and cell wall compositional changes were analyzed. Arabinose proved to be the principal neutral monosaccharide constituent in cell walls during growth and the most dynamic neutral sugar in pectic fractions. Arabinose loss from tightly bound pectins was found to be a relatively early feature in the sequence of cell wall biochemical modifications, thus suggesting a softening-related role during Japanese plum on-tree ripening. Depolymerization of matrix glycans started at the end of the cell expansion phase and increased throughout ripening. Pectin solubilization was first detected during early ripening. Firmness loss did not correlate with polyuronide depolymerization early in ripening, but the last softening phase was associated with a strong depolymerization of cell wall polyuronides as well as a decrease in the arabinose/galactose ratio in loosely bound pectins. This is the first work that characterizes the temporal sequence of cell wall polysaccharide changes in Japanese plum.
采自六个发育阶段的日本李，无中间贮藏期，分析细胞壁组成变化。在生长过程中阿拉伯糖被证明是细胞壁中主要的中性单糖成分，也是果胶部分最具动态的中性糖。从紧密结合的果胶中损失阿拉伯糖被认为是细胞壁生化修饰序列中的一个相对早期特征，因此在日本李树上成熟过程中具有软化相关作用。基质聚糖的解聚始于细胞扩展阶段结束时，并在整个成熟过程中增加。果胶的溶解最早在成熟早期被检测到。在成熟早期，硬度损失与聚半乳糖醛酸解聚没有相关性，但最后一个软化阶段与细胞壁聚半乳糖醛酸的强烈解聚以及松散结合的果胶中阿拉伯糖/半乳糖比例的降低有关。这是首次描述日本李细胞壁多糖变化的时间顺序。
