Department of Biosciences, School of Science, Kitasato University, 1-15-1 Sagamihara, Kanagawa 228-8555, Japan.
Immunopharmacol Immunotoxicol. 2010 Sep;32(3):454-61. doi: 10.3109/08923970903513139.
To investigate the antiallergic effects of fermented grape marc from Negroamaro (N-FGM), we examined antigen (Ag)-induced degranulation of rat basophilic leukemia (RBL-2H3) cells. Among supernatants of N-FGM suspensions in water, ethanol, and dimethyl sulfoxide (DMSO), supernatants of DMSO-suspended N-FGM but not of nonfermented Negroamaro grape marc (N-GM) markedly suppressed the Ag-induced degranulation and phosphorylation of Syk in RBL-2H3 cells. Supernatants of DMSO-suspended N-FGM did not reduce the expression of FcepsilonRI on RBL-2H3 cells. Analyses of supernatants of N-FGM suspensions in water, ethanol, and DMSO by high-performance liquid chromatography revealed higher amounts of quercetin in supernatants of DMSO-suspended N-FGM than those in the other supernatants. Quercetin also suppressed the Ag-induced degranulation and phosphorylation of Syk but did not reduce the expression of FcepsilonRI on RBL-2H3 cells. These results suggest that inhibition of the Ag-induced degranulation and Syk phosphorylation by N-FGM might be due to the action of quercetin, as an active component in N-FGM.
为了研究黑珍珠（ Negroamaro ）发酵葡萄渣（ N-FGM ）的抗过敏作用，我们检测了 N-FGM 悬浮液（水、乙醇和二甲基亚砜 DMSO ）、N-GM 悬浮液对大鼠嗜碱性白血病细胞（ RBL-2H3 ）脱颗粒和 Syk 磷酸化的影响。结果表明，DMSO 悬浮的 N-FGM 而非未发酵的黑珍珠葡萄渣（ N-GM ）可显著抑制 RBL-2H3 细胞的脱颗粒和 Syk 磷酸化。DMSO 悬浮的 N-FGM 不降低 RBL-2H3 细胞表面 FcepsilonRI 的表达。高效液相色谱分析显示，DMSO 悬浮的 N-FGM 上清液中槲皮素含量高于其他上清液。槲皮素也可抑制 Ag 诱导的脱颗粒和 Syk 磷酸化，但不降低 RBL-2H3 细胞表面 FcepsilonRI 的表达。这些结果表明，N-FGM 抑制 Ag 诱导的脱颗粒和 Syk 磷酸化可能与 N-FGM 中的槲皮素等活性成分有关。
