Department of Animal Biology, University of Bucharest, Romania.
Microsc Microanal. 2010 Apr;16(2):137-42. doi: 10.1017/S1431927609991346. Epub 2010 Jan 26.
In this study the structure of collagen-chondroitin sulphate-hydroxyapatite porous composites is investigated by histochemical (Von Kossa staining), immunohistochemical, and transmission electron microscopy. Examination of composites on picrosirius red stained sections showed that polarization colors of collagen were generally in the range of orange-red. Immunofluorescence data indicate that chondroitin sulphate was either chemically incorporated into the bulk structure of collagen scaffolds or attached on surfaces of collagen bundles. Depending on the ratio between collagen:chondroitin sulphate:hydroxyapatite, von Kossa histochemical staining showed a progressive loading of collagen-chondroitin sulphate bundles with hydroxyapatite. Transmission electron microscopy studies have shown that composites contain mostly collagen fibrils aggregated with random orientation with very few collagen fibers showing the 67-nm banding pattern. Hydroxyapatite deposits of various sizes occurred among the collagen fibrils.
本研究通过组织化学（Von Kossa 染色）、免疫组织化学和透射电子显微镜观察了胶原-硫酸软骨素-羟基磷灰石多孔复合材料的结构。对 picrosirius 红染色切片上的复合材料进行检查表明，胶原的偏光颜色通常在橙红色范围内。免疫荧光数据表明，硫酸软骨素要么化学掺入胶原支架的主体结构中，要么附着在胶原束的表面上。根据胶原：硫酸软骨素：羟基磷灰石的比例，Von Kossa 组织化学染色显示胶原-硫酸软骨素束逐渐负载羟基磷灰石。透射电子显微镜研究表明，复合材料主要包含胶原纤维，这些纤维以随机方向聚集，很少有胶原纤维显示出 67nm 的带型。在胶原纤维之间存在各种大小的羟基磷灰石沉积物。
