大鼠单侧迷路切除术后前庭代偿期间前庭-胃肠症状的恢复。

Department of Physiology, Wonkwang University School of Medicine, Vestibulocochlear Research Center & Brain Research Institute at Wonkwang University, Iksan, Korea.

Otol Neurotol. 2010 Feb;31(2):241-9. doi: 10.1097/MAO.0b013e3181cabd5d.

BACKGROUND

The loss of unilateral vestibular function causes vestibulogastrointestinal symptoms that include nausea and vomiting. However, the temporal changes occurring on vestibular compensation are unclear. Thus, the temporal changes and the role of the cerebellum in the recovery of vestibulogastrointestinal symptoms after unilateral labyrinthectomy (UL) were investigated in this study.

METHODS

Vestibulogastrointestinal symptoms were evaluated for intestinal transit and geometric center, whereas vestibulo-ocular symptoms were represented by spontaneous nystagmus. Expression of the c-Fos protein was observed in the vestibular nuclei. These were measured at 30 minutes and at 2, 6, and 24 hours after UL in rats.

RESULTS

Intestinal transit was 66.3% +/- 7.6% in the control animals but significantly decreased to 40.7% +/- 7.8%, 46.3% +/- 6.3%, and 48.6% +/- 10.8% at 30 minutes (p < 0.01), 2 hours (p < 0.01), and 6 hours (p < 0.05) after UL, respectively. The intestinal transit showed a recovery to control levels 24 hours after UL. The geometric center was 5.6 +/- 0.4 in control animals but significantly decreased to 2.1 +/- 0.4, 2.9 +/- 0.3, and 4.0 +/- 0.3 at 30 minutes, 2 hours, and 6 hours after UL, respectively (p < 0.01). Recovery of the geometric center to control levels, 24 hours after UL, was reported. Uvulonodullectomy significantly decreased the intestinal transit and geometric center for 24 hours after surgery (p < 0.01). Moreover, UL in uvulonodullectomized animals significantly decreased the intestinal transit and geometric center for 24 hours after surgery (p < 0.01). Pretreatment of the UL animals with MK-801 significantly increased the geometric center 30 minutes after surgery (p < 0.01). Unilateral labyrinthectomy produced spontaneous nystagmus, 28.9 +/- 1.5, 23.3 +/- 1.4, 17.5 +/- 1.5, and 9.2 +/- 0.9 beats per 10 seconds at 30 minutes and at 2, 6, and 24 hours after UL, respectively. Expression of the c-Fos protein was significantly increased in the medial vestibular nuclei and inferior vestibular nuclei at 1, 2, and 6 hours after UL, and the expression was significantly decreased in animals that were pretreated with MK-801 (p < 0.01).

CONCLUSION

These results suggest that the recovery of vestibulogastrointestinal symptoms is faster than that of vestibulo-ocular symptoms and that the cerebellum and glutamate have an important role to play in the recovery of symptoms after UL.

背景

单侧前庭功能丧失会导致前庭胃肠症状，包括恶心和呕吐。然而，前庭代偿过程中的时间变化尚不清楚。因此，本研究旨在探讨单侧迷路切除（UL）后前庭胃肠症状恢复过程中的时间变化以及小脑的作用。

方法

通过肠道传输和几何中心评估前庭胃肠症状，通过自发性眼球震颤评估前庭眼症状。在大鼠 UL 后 30 分钟、2 小时、6 小时和 24 小时观察前庭核内 c-Fos 蛋白的表达。

结果

对照组动物的肠道传输率为 66.3% +/- 7.6%，但在 UL 后 30 分钟（p < 0.01）、2 小时（p < 0.01）和 6 小时（p < 0.05）分别显著下降至 40.7% +/- 7.8%、46.3% +/- 6.3%和 48.6% +/- 10.8%。UL 后 24 小时，肠道传输率恢复至对照水平。对照组动物的几何中心为 5.6 +/- 0.4，但在 UL 后 30 分钟、2 小时和 6 小时分别显著下降至 2.1 +/- 0.4、2.9 +/- 0.3 和 4.0 +/- 0.3（p < 0.01）。UL 后 24 小时，几何中心恢复至对照水平。手术后，行扁桃体和卵圆窝切除术（uvulonodullectomy）显著降低了肠道传输率和几何中心（p < 0.01）。此外，在扁桃体和卵圆窝切除术动物中，UL 后 24 小时也显著降低了肠道传输率和几何中心（p < 0.01）。UL 动物的 MK-801 预处理显著增加了术后 30 分钟的几何中心（p < 0.01）。单侧迷路切除术后产生的自发性眼球震颤分别为 28.9 +/- 1.5、23.3 +/- 1.4、17.5 +/- 1.5 和 9.2 +/- 0.9 次/10 秒，在 UL 后 30 分钟以及 2、6 和 24 小时分别观察到。UL 后 1、2 和 6 小时，内侧前庭核和下前庭核内 c-Fos 蛋白的表达显著增加，MK-801 预处理的动物表达显著减少（p < 0.01）。