Kim M S, Jin B K, Chun S W, Lee M Y, Lee S H, Kim J H, Park B R
Department of Physiology, Wonkwang University School of Medicine and Medicinal Resources Research Center of Wonkwang University, Iksan, South Korea.
Neurosci Lett. 1997 Aug 15;231(3):147-50. doi: 10.1016/s0304-3940(97)00550-8.
The purpose of this study was to evaluate the effect of uvulonodullectomy (UNL) on the expression of cFos-like protein (FLP) in the medial vestibular nucleus (MVe) during vestibular compensation and effect of MK801, an N-methyl-D-aspartate (NMDA) antagonist, on FLP expression in the brain stem nuclei at 6 h after unilateral labyrinthectomy (ULX) with UNL in Sprague-Dawley rats. Immunohistochemical staining was performed to visualize FLP in the brain stem nuclei and FLP-positive cells were counted by image analyzer. Lesion-induced asymmetric expression of FLP in the bilateral MVe was observed and maintained up to for 72 h in the ULX group, and 120 h in the UNL + ULX group. Moreover, spatial pattern of FLP expression in the bilateral MVe exhibited the marked difference between the ULX and UNL + ULX groups. MK801 treatment 6 h after ULX showed significant increase in the number of FLP in contralateral MVe (cMVe) of the ULX group, but decrease in cMVe of the UNL + ULX group. These results suggest that the lesion of vestibulocerebellum delays the temporal recovery of FLP expression in MVe and the vestibulocerebellar NMDA receptors relate to FLP expression in MVe.
本研究的目的是评估悬雍垂球囊切除术（UNL）对前庭代偿期间内侧前庭核（MVe）中cFos样蛋白（FLP）表达的影响，以及N-甲基-D-天冬氨酸（NMDA）拮抗剂MK801对Sprague-Dawley大鼠单侧迷路切除术（ULX）联合UNL术后6小时脑干核团中FLP表达的影响。采用免疫组织化学染色法观察脑干核团中的FLP，并通过图像分析仪对FLP阳性细胞进行计数。在ULX组中观察到损伤诱导的双侧MVe中FLP不对称表达，并持续72小时，在UNL + ULX组中持续120小时。此外，双侧MVe中FLP表达的空间模式在ULX组和UNL + ULX组之间表现出明显差异。ULX术后6小时给予MK801治疗，ULX组对侧MVe（cMVe）中FLP数量显著增加，但UNL + ULX组cMVe中FLP数量减少。这些结果表明，前庭小脑损伤延迟了MVe中FLP表达的时间恢复，并且前庭小脑NMDA受体与MVe中FLP表达有关。
