Max-Planck-Institut fuer Molekulare Pflanzenphysiologie, Am Muehlenberg 1, 14476 Potsdam, Germany.
Protoplasma. 2010 May;241(1-4):29-36. doi: 10.1007/s00709-009-0099-7. Epub 2010 Jan 26.
Gene expression levels of several transcription factors from Arabidopsis thaliana that were described previously to be involved in leaf development and trichome formation were analysed in trichome, basal and pavement cells of mature leaves. Single cell samples of these three cells types were collected by glass micro-capillaries. Real-time reverse transcription (RT)-PCR was used to analyse expression patterns of the following transcription factors: MYB23, MYB55, AtHB1, FILAMENTOUS FLOWER (FIL)/YABBY1 (YAB1), TRIPTYCHON (TRY) and CAPRICE (CPC). A difference in the expression patterns of TRY and CPC was revealed. Contrary to the CPC expression pattern, no transcripts of TRY could be detected in pavement cells. FIL/YAB1 was exclusively expressed in trichome cells. AtHB1 was highly expressed throughout all three cell types. MYB55 was higher expressed in basal cells than in trichome and pavement cells. MYB23 showed a pattern of low expression in pavement cells, medium in basal cells and high expression in trichomes. Expression patterns obtained by single cell sampling and real-time RT-PCR were compared to promoter GUS fusions of the selected transcription factors. Therefore, we regenerated two transgenic Arabidopsis lines that expressed the GUS reporter gene under control of the promoters of MYB55 and YAB1. In conclusion, despite their function in leaf morphogenesis, all six transcription factors were detected in mature leaves. Furthermore, single cell sampling and promoter GUS staining patterns demonstrated the predominant presence of MYB55 in basal cells as compared to pavement cells and trichomes.
先前有研究报道，拟南芥中几种与叶片发育和毛状体形成有关的转录因子的基因表达水平在成熟叶片的毛状体、基部和表皮细胞中进行了分析。通过玻璃微管收集这三种细胞类型的单细胞样本。采用实时 RT-PCR 分析了以下转录因子的表达模式：MYB23、MYB55、AtHB1、FILAMENTOUS FLOWER（FIL）/YABBY1（YAB1）、TRIPTYCHON（TRY）和CAPRICE（CPC）。揭示了 TRY 和 CPC 的表达模式存在差异。与 CPC 的表达模式相反，在表皮细胞中无法检测到 TRY 的转录本。FIL/YAB1 仅在毛状体细胞中表达。AtHB1 在所有三种细胞类型中均高度表达。MYB55 在基部细胞中的表达高于毛状体和表皮细胞。MYB23 在表皮细胞中的表达水平较低，在基部细胞中的表达水平中等，在毛状体中的表达水平较高。通过单细胞取样和实时 RT-PCR 获得的表达模式与选定转录因子的启动子 GUS 融合进行了比较。因此，我们再生了两个转基因拟南芥系，它们在 MYB55 和 YAB1 的启动子控制下表达 GUS 报告基因。总之，尽管这六种转录因子在叶片形态发生中具有功能，但在成熟叶片中均有检测到。此外，单细胞取样和启动子 GUS 染色模式表明，与毛状体和表皮细胞相比，MYB55 在基部细胞中主要存在。
