在肢体关节发育过程中 versican 的蛋白水解裂解。
Proteolytic cleavage of versican during limb joint development.
机构信息
Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA.
出版信息
Anat Rec (Hoboken). 2010 Feb;293(2):208-14. doi: 10.1002/ar.21049.
Abstract
Versican is highly expressed in developing joint interzones during limb morphogenesis. This study was undertaken to examine whether proteolytic cleavage of versican occurs that could potentially impact its function during the process of embryonic synovial joint formation. Using an antibody to the DPEAAE neoepitope generated by ADAMTS proteolysis, versican amino terminal cleavage fragments were detected in joint interzones at 12-16 days post coitum (dpc). ADAMTS-1 localization overlapped that of DPEAAE-reactive versican fragments suggesting it as one possible protease activity involved in processing of versican in the interzone. Results show that increased cleavage of versican in the interzone accompanies cavitation and suggests that proteolytic modification of versican may be important during the process of synovial joint maturation.
摘要
在肢体形态发生过程中，软骨钙素在发育中的关节间区高度表达。本研究旨在探讨软骨钙素是否发生蛋白水解切割，这可能会影响其在胚胎滑膜关节形成过程中的功能。使用针对 ADAMTS 蛋白水解产生的 DPEAAE 新表位的抗体，在 12-16 天龄胎鼠（dpc）的关节间区检测到软骨钙素氨基末端切割片段。ADAMTS-1 的定位与 DPEAAE 反应性软骨钙素片段重叠，表明其可能是参与间区软骨钙素加工的一种潜在蛋白酶活性。结果表明，间区软骨钙素的切割增加伴随着囊泡形成，提示蛋白水解修饰软骨钙素可能在滑膜关节成熟过程中很重要。
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