Breast Center, Department of Medicine and Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Contrast Media Mol Imaging. 2010 Jan-Feb;5(1):34-8. doi: 10.1002/cmmi.293.
Gadonanotubes (GNTs), which are powerful new T(1)-weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water-soluble (2 mg ml(-1)) ser-gadonanotubes (ser-GNs) as magnetic nanoprobes for intracellular labeling. The ser-GNTs were used to efficiently label MCF-7 human breast cancer cells (1.5 x 10(9) Gd(3+) ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser-GNT labeled cells give bright T(1)-weighted MR images, confirming that the ser-GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking.
甘氨酰胺纳米管（GNTs）是一种强大的新型 T1 加权磁共振成像对比剂，通过引入丝氨酸氨基酸取代基，将其转化为水溶性（2mg/ml）的丝氨酸甘氨酰胺纳米管（ser-GNTs），作为用于细胞内标记的磁性纳米探针。ser-GNTs 可有效地标记 MCF-7 人乳腺癌细胞（1.5×109个 Gd3+离子/细胞），且无明显细胞毒性。来源于 ser-GNT 标记细胞的细胞沉淀给出了明亮的 T1 加权磁共振图像，证实了 ser-GNTs 是一种有前途的新型纳米探针技术，可用于磁性细胞标记，并且可能用于体内细胞转运。
