National Taiwan University College of Medicine and Hospital, Taipei, Taiwan.
J Am Chem Soc. 2010 Feb 17;132(6):1744-5. doi: 10.1021/ja9086038.
The ability to generate antimicrobial reactive-oxygen species (ROS) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for the host to defend against invading microbes. We have demonstrated the application of confocal autofluorescence microscopy and Raman microspectroscopy to characterize dynamically the phagocytic process of living macrophages in a label-free manner. In particular, we visualized the translocation of NADPH oxidases in living macrophages that are undergoing phagocytosis of invading yeasts and monitored dynamically the change at the molecular level of single intraphagosomal yeasts caused by phagocytic ROS.
还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶产生抗微生物活性氧（ROS）的能力对于宿主抵御入侵微生物至关重要。我们已经证明了共聚焦自发荧光显微镜和拉曼微光谱学的应用，可以以非标记的方式动态表征活巨噬细胞的吞噬过程。特别是，我们可视化了活巨噬细胞中 NADPH 氧化酶在吞噬入侵酵母时的易位，并动态监测吞噬 ROS 引起的单个吞噬体酵母内部分子水平的变化。
