Division of Human Gene Therapy, Department of Medicine, Gene Therapy Center, University of Alabama at Birmingham, AL 35294, USA.
Virol J. 2010 Jan 26;7:21. doi: 10.1186/1743-422X-7-21.
Adenoviral vectors have been utilized for a variety of gene therapy applications. Our group has incorporated bioluminescent, fluorographic reporters, and/or suicide genes within the adenovirus genome for analytical and/or therapeutic purposes. These molecules have also been incorporated as capsid components. Recognizing that incorporations at either locale yield potential advantages and disadvantages, our report evaluates the benefits of transgene incorporation versus capsid incorporation. To this end, we have genetically incorporated firefly luciferase within the early region 3 or at minor capsid protein IX and compared vector functionality by means of reporter readout.
腺病毒载体已被广泛应用于各种基因治疗领域。我们的团队已将生物发光、荧光报告基因和/或自杀基因整合到腺病毒基因组中,以达到分析和/或治疗的目的。这些分子也被整合到衣壳成分中。鉴于在这两个位置进行整合都可能带来优势和劣势,我们的报告评估了转基因整合与衣壳整合的优缺点。为此,我们在早期区域 3 或次要衣壳蛋白 IX 中进行了萤火虫荧光素酶的基因整合,并通过报告基因读出比较了载体的功能。