Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via per Arnesano Km 1, 73100 Lecce, Italy.
Anal Chim Acta. 2010 Feb 5;659(1-2):167-71. doi: 10.1016/j.aca.2009.11.046. Epub 2009 Nov 27.
A highly selective molecularly imprinted polymer (MIP) for 1-methyladenosine (1-MA), an urinary modified nucleoside used as cancer marker, was prepared and its use as solid-phase extraction (SPE) sorbent material was demonstrated. The MIP was prepared by a very simple procedure using methacrylic acid as functional monomer and a mixture acetonitrile/water (4/1, v/v) as porogen, overcoming in this way the problems usually related to the imprinting of biological polar compounds. The MIP was tested in batch experiments in order to evaluate its binding properties and then used as SPE sorbent for the selective clean-up and pre-concentration of 1-MA. The extraction protocol was successfully applied to the direct extraction of 1-MA from spiked human urine indicating that the MIP allowed 1-MA to be pre-concentrated while simultaneously interfering compounds were removed from the matrix.
一种高选择性的 1-甲基腺苷（1-MA）分子印迹聚合物（MIP）被制备，1-MA 是一种用于癌症标志物的尿液修饰核苷。该 MIP 是通过一种非常简单的方法制备的，使用甲基丙烯酸作为功能单体，乙腈/水（4/1，v/v）混合物作为致孔剂，从而克服了通常与生物极性化合物印迹相关的问题。MIP 在批量实验中进行了测试，以评估其结合性能，然后用作 SPE 吸附剂，用于选择性地净化和预浓缩 1-MA。该提取方案成功地应用于从加标人尿中直接提取 1-MA，表明 MIP 允许在浓缩 1-MA 的同时，从基质中去除干扰化合物。
