Department of Chemistry, Amirkabir University of Technology, Tehran, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 15;878(20):1700-6. doi: 10.1016/j.jchromb.2010.04.006. Epub 2010 Apr 24.
In this study, a novel method is described for the determination of tramadol in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and tramadol as template molecule. The novel imprinted polymer was used as a solid-phase extraction (SPE) sorbent for the extraction of tramadol from human plasma and urine. Various parameters affecting the extraction efficiency of the polymer have been evaluated. The optimal conditions for the MIP cartridges were studied. The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of tramadol. The limit of detection (LOD) and limit of quantification (LOQ) for tramadol in urine samples were 1.2 and 3.5 microg L(-1), respectively. These limits for tramadol in plasma samples were 3.0 and 8.5 microg L(-1), respectively. The recoveries for plasma and urine samples were higher than 91%.
在这项研究中，描述了一种使用分子印迹固相萃取（MISPE）作为样品净化技术结合高效液相色谱（HPLC）来测定生物流体中曲马多的新方法。采用甲基丙烯酸作为功能单体、乙二醇二甲基丙烯酸酯作为交联剂、氯仿作为致孔剂、曲马多作为模板分子，制备了亲水性分子印迹聚合物（MIPs）。新型印迹聚合物被用作固相萃取（SPE）吸附剂，从人血浆和尿液中提取曲马多。评估了影响聚合物萃取效率的各种参数。研究了 MIP 小柱的最佳条件。通过检查几种与曲马多具有相似分子结构的物质来评估 MIP 的选择性。尿液样品中曲马多的检测限（LOD）和定量限（LOQ）分别为 1.2 和 3.5 μg L(-1)。血浆样品中曲马多的检测限和定量限分别为 3.0 和 8.5 μg L(-1)。血浆和尿液样品的回收率均高于 91%。
