• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

猪源转座子介导的小鼠基因的长期表达。

piggyBac transposon-mediated long-term gene expression in mice.

机构信息

Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi-cho, Kyoto, Japan.

出版信息

Mol Ther. 2010 Apr;18(4):707-14. doi: 10.1038/mt.2009.302. Epub 2010 Jan 26.

DOI:10.1038/mt.2009.302
PMID:20104210
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2862518/
Abstract

Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection methods. piggyBac (PB) transposon has a high transposability in mammalian cells in vitro, and has been used for genetic and preclinical studies. However, the transposability of PB in mammalian somatic cells in vivo has not been demonstrated yet. Here, we demonstrated PB-mediated sustained gene expression in adult mice. We constructed PB-based plasmid DNA (pDNA) containing reporter [firefly and Gaussia luciferase (Gluc)] genes. Mice were transfected by injection of these pDNAs using a hydrodynamics-based procedure, and the conditions for high-level sustained gene expression were examined. Consequently, gene expressions were sustained over 2 months. Our results suggest that PB is useful for organ-selective somatic integration and sustained gene expression in mammals, and will contribute to basic genetic studies and gene therapies.

摘要

转座子是一种很有前途的体基因整合系统，因为它们不仅可以有效地整合外源基因，而且可以使用多种转染方法将其递送到各种器官。piggyBac (PB) 转座子在体外的哺乳动物细胞中转座活性很高，已被用于遗传和临床前研究。然而，PB 在哺乳动物体细胞核内的转座活性尚未得到证实。在这里，我们证明了 PB 介导的成年小鼠中的持续基因表达。我们构建了基于 PB 的质粒 DNA（pDNA），其中包含报告基因[萤火虫荧光素酶和高斯荧光素酶（Gluc）]。通过基于流体动力学的程序注射这些 pDNAs 对小鼠进行转染，并检查了高水平持续基因表达的条件。结果，基因表达持续了 2 个月以上。我们的结果表明，PB 可用于哺乳动物的器官选择性体基因整合和持续基因表达，并将有助于基础遗传研究和基因治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/1dbb2ead9436/mt2009302f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/cb5033dfa4d7/mt2009302f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/808568843289/mt2009302f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/726403600a4e/mt2009302f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/3fb6d5f2c56e/mt2009302f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/2824ff6a0e53/mt2009302f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/a6c37731ae4d/mt2009302f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/1dbb2ead9436/mt2009302f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/cb5033dfa4d7/mt2009302f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/808568843289/mt2009302f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/726403600a4e/mt2009302f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/3fb6d5f2c56e/mt2009302f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/2824ff6a0e53/mt2009302f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/a6c37731ae4d/mt2009302f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/2862518/1dbb2ead9436/mt2009302f7.jpg

相似文献

1
piggyBac transposon-mediated long-term gene expression in mice.猪源转座子介导的小鼠基因的长期表达。
Mol Ther. 2010 Apr;18(4):707-14. doi: 10.1038/mt.2009.302. Epub 2010 Jan 26.
2
Intravenous Delivery of Bac Transposons as a Useful Tool for Liver-Specific Gene-Switching.静脉注射 Bac 转座子作为一种用于肝脏特异性基因开关的有用工具。
Int J Mol Sci. 2018 Nov 2;19(11):3452. doi: 10.3390/ijms19113452.
3
In Vivo bac-Based Gene Delivery towards Murine Pancreatic Parenchyma Confers Sustained Expression of Gene of Interest.体内基于细菌的基因传递方法向小鼠胰腺实质传递目的基因，可实现目的基因的持续表达。
Int J Mol Sci. 2019 Jun 26;20(13):3116. doi: 10.3390/ijms20133116.
4
[Development and therapeutic application of transposon-based vectors].[基于转座子的载体的开发与治疗应用]
Yakugaku Zasshi. 2009 Dec;129(12):1433-43. doi: 10.1248/yakushi.129.1433.
5
High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts.猪内源性转座子介导的高水平转基因表达增强宫颈癌异种移植体中的转基因治疗效果。
Oncol Rep. 2010 Oct;24(4):897-907. doi: 10.3892/or.2010.897.
6
Integration and long-term expression in xenografted human glioblastoma cells using a plasmid-based transposon system.使用基于质粒的转座子系统在异种移植的人胶质母细胞瘤细胞中的整合和长期表达。
Mol Ther. 2004 Aug;10(2):260-8. doi: 10.1016/j.ymthe.2004.05.005.
7
PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer.经体细胞核移植后，基于 PiggyBac 转座子的诱导性基因表达。
Mol Ther. 2009 Dec;17(12):2115-20. doi: 10.1038/mt.2009.234. Epub 2009 Oct 6.
8
Tumor-directed gene therapy in mice using a composite nonviral gene delivery system consisting of the piggyBac transposon and polyethylenimine.使用由猪尾巴转座子和聚乙烯亚胺组成的复合非病毒基因递送系统在小鼠中进行肿瘤导向基因治疗。
BMC Cancer. 2009 Apr 27;9:126. doi: 10.1186/1471-2407-9-126.
9
Transposons: Moving Forward from Preclinical Studies to Clinical Trials.转座子：从临床前研究迈向临床试验。
Hum Gene Ther. 2017 Nov;28(11):1087-1104. doi: 10.1089/hum.2017.128. Epub 2017 Aug 22.
10
Evaluation of long-term gene expression in mouse liver using PhiC31 integrase and hydrodynamic injection.利用 PhiC31 整合酶和水力注射评估小鼠肝脏中的长期基因表达。
Biol Pharm Bull. 2012;35(7):1182-6. doi: 10.1248/bpb.b12-00083.

引用本文的文献

1
Developing anti-TDE vaccine for sensitizing cancer cells to treatment and metastasis control.开发抗TDE疫苗，以使癌细胞对治疗敏感并控制转移。
NPJ Vaccines. 2025 Jan 27;10(1):18. doi: 10.1038/s41541-024-01035-3.
2
Bioluminescent Systems for Theranostic Applications.用于治疗应用的生物发光系统。
Int J Mol Sci. 2024 Jul 10;25(14):7563. doi: 10.3390/ijms25147563.
3
High-throughput functional screen identifies YWHAZ as a key regulator of pancreatic cancer metastasis.高通量功能筛选鉴定 YWHAZ 为胰腺癌转移的关键调节因子。

本文引用的文献

1
Pressure-mediated transfection of murine spleen and liver.压力介导的小鼠脾脏和肝脏转染。
Hum Gene Ther. 2009 Oct;20(10):1157-67. doi: 10.1089/hum.2008.213.
2
Sleeping beauty transposition from nonintegrating lentivirus.来自非整合慢病毒的睡美人转座
Mol Ther. 2009 Jul;17(7):1197-204. doi: 10.1038/mt.2009.94. Epub 2009 May 5.
3
Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates.新型超活性 Sleeping Beauty 转座酶的分子进化使脊椎动物中强大稳定的基因转移成为可能。
Cell Death Dis. 2023 Jul 14;14(7):431. doi: 10.1038/s41419-023-05951-5.
4
In Utero Electroporation for Manipulation of Specific Neuronal Populations.用于操纵特定神经元群体的子宫内电穿孔
Membranes (Basel). 2022 May 11;12(5):513. doi: 10.3390/membranes12050513.
5
Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock.Gpr19 是一种生物钟控制的孤儿 GPCR，在调节生物钟的自由运行周期和光重置能力方面发挥作用。
Sci Rep. 2021 Nov 17;11(1):22406. doi: 10.1038/s41598-021-01764-8.
6
Conditioned CAR-T cells by hypoxia-inducible transcription amplification (HiTA) system significantly enhances systemic safety and retains antitumor efficacy.缺氧诱导转录扩增（HiTA）系统条件化的 CAR-T 细胞可显著提高系统安全性并保持抗肿瘤疗效。
J Immunother Cancer. 2021 Oct;9(10). doi: 10.1136/jitc-2021-002755.
7
Novel vectors and approaches for gene therapy in liver diseases.用于肝病基因治疗的新型载体和方法。
JHEP Rep. 2021 Apr 30;3(4):100300. doi: 10.1016/j.jhepr.2021.100300. eCollection 2021 Aug.
8
Understanding In Vivo Fate of Nucleic Acid and Gene Medicines for the Rational Design of Drugs.了解核酸和基因药物的体内命运以进行药物的合理设计。
Pharmaceutics. 2021 Jan 26;13(2):159. doi: 10.3390/pharmaceutics13020159.
9
Use of a Hybrid Adeno-Associated Viral Vector Transposon System to Deliver the Insulin Gene to Diabetic NOD Mice.使用腺相关病毒载体转座子系统将胰岛素基因递送至糖尿病 NOD 小鼠。
Cells. 2020 Oct 2;9(10):2227. doi: 10.3390/cells9102227.
10
The Evolution of Gene Therapy in the Treatment of Metabolic Liver Diseases.基因治疗在代谢性肝病治疗中的发展。
Genes (Basel). 2020 Aug 10;11(8):915. doi: 10.3390/genes11080915.
Nat Genet. 2009 Jun;41(6):753-61. doi: 10.1038/ng.343. Epub 2009 May 3.
4
Tumor-directed gene therapy in mice using a composite nonviral gene delivery system consisting of the piggyBac transposon and polyethylenimine.使用由猪尾巴转座子和聚乙烯亚胺组成的复合非病毒基因递送系统在小鼠中进行肿瘤导向基因治疗。
BMC Cancer. 2009 Apr 27;9:126. doi: 10.1186/1471-2407-9-126.
5
Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo.用于监测体外培养和体内生物过程的海肾荧光素酶报告基因检测。
Nat Protoc. 2009;4(4):582-91. doi: 10.1038/nprot.2009.28.
6
Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon.利用piggyBac转座子产生无转基因的诱导多能小鼠干细胞。
Nat Methods. 2009 May;6(5):363-9. doi: 10.1038/nmeth.1323. Epub 2009 Mar 31.
7
piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.piggyBac转座将成纤维细胞重编程为诱导多能干细胞。
Nature. 2009 Apr 9;458(7239):766-70. doi: 10.1038/nature07863. Epub 2009 Mar 1.
8
Virus-free induction of pluripotency and subsequent excision of reprogramming factors.无病毒诱导多能性及随后重编程因子的切除
Nature. 2009 Apr 9;458(7239):771-5. doi: 10.1038/nature07864. Epub 2009 Mar 1.
9
Hybrid lentivirus-transposon vectors with a random integration profile in human cells.在人类细胞中具有随机整合模式的混合慢病毒-转座子载体。
Mol Ther. 2009 Jul;17(7):1205-14. doi: 10.1038/mt.2009.10. Epub 2009 Feb 24.
10
De novo induction of genetically engineered brain tumors in mice using plasmid DNA.利用质粒DNA在小鼠中从头诱导基因工程脑肿瘤
Cancer Res. 2009 Jan 15;69(2):431-9. doi: 10.1158/0008-5472.CAN-08-1800.