Department of Animal and Human Biology, University of Turin, Turin, Italy.
J Neurosci Methods. 2010 Apr 30;188(1):71-5. doi: 10.1016/j.jneumeth.2010.01.024. Epub 2010 Jan 25.
The employment of transgenic mouse models for peripheral nerve regeneration studies is continuously increasing. In this paper, we describe a standardized method for inducing a crush lesion in mouse median nerve using a non-serrated clamp exerting a crush compression force of 20.43 MPa for a duration of 30 s. Quantitative assessment of posttraumatic functional recovery by grasping test showed that recovery was very fast and mice returned to baseline performance already after 20 days only. Stereological analysis of nerve fibers distal to the crush lesion showed the presence of axons with a significantly smaller size and thinner myelin sheath in comparison to controls. This experimental nerve injury model is highly reproducible and the impact on animal well-being is minimal. Its employment can be particularly indicated for exploring the basic neurobiological mechanisms of peripheral nerve regeneration.
在周围神经再生研究中，转基因小鼠模型的应用不断增加。本文描述了一种使用非锯齿夹在小鼠正中神经上施加 20.43 MPa 挤压压力 30 秒来诱导挤压损伤的标准化方法。通过抓取试验对创伤后功能恢复进行定量评估表明，恢复非常迅速，小鼠仅在 20 天后就恢复到基线水平。对挤压损伤远端神经纤维的体视学分析显示，与对照组相比，存在轴突尺寸明显更小且髓鞘更薄的轴突。这种实验性神经损伤模型具有高度可重复性，对动物福利的影响很小。它的应用特别适合于探索周围神经再生的基本神经生物学机制。
