Setiadi H, Antoine C, Wautier J L
Institut des Vaisseaux et du Sang (IVS) Hôpital Lariboisière, Paris.
J Mal Vasc. 1991;16(1):30-1.
The glycoprotein complex GPIIb-IIIa of the platelet membrane and CD18 of the monocyte membrane have been established as being the central figure for the adhesion processes of the corresponding cells. The molecular structure of these 2 GPs bears some similarities. It was proposed recently that LFA-1 and Mo-1 (CD18 family) and GPIIb-IIIa might be encoded by the same gene. For this purpose, we studied the expression of Mo-1, Mo-2 (as control) receptors on monocytes and GPIIIa and GPIIb-IIIa on platelets of two GT patients as compared to normal healthy subjects. Monoclonal antibodies anti Mo-1, MO-2, AP-3 and AP-2 were used to measure the expression of the respective antigens by indirect immunofluorescence procedure. The fluorescence of the labelled cells was analysed with an Ortho Cytofluorograph 50-H. The resulting Mean Fluorescence Intensity (MFI) values of AP-2 and AP-3 showed that the patients had a total absence of GPIIb-IIIa antigens. However, Mo-1, as well as those of control Mo-2, in patients (MO-1: 672 and 716; Mo-2: 453 and 637) were similar to that of normal subjects (Mo-1: 735 +/- 74; Mo-2: 585 +/- 35; mean +/- SEM). Therefore, the normal expression of Mo-1 receptors on the monocytes of Glanzmann's thrombasthenia patients suggests different genomic regulations for Mo-1 antigens on the monocyte and GPIIb-IIIa complex on the platelet.
血小板膜糖蛋白复合物GPIIb-IIIa和单核细胞膜CD18已被确认为相应细胞黏附过程的核心物质。这两种糖蛋白的分子结构有一些相似之处。最近有人提出，淋巴细胞功能相关抗原-1（LFA-1）和巨噬细胞抗原-1（Mo-1，CD18家族）以及GPIIb-IIIa可能由同一基因编码。为此，我们研究了两名血小板无力症（GT）患者与正常健康受试者相比，单核细胞上Mo-1、Mo-2（作为对照）受体以及血小板上GPIIIa和GPIIb-IIIa的表达情况。使用抗Mo-1、MO-2、AP-3和AP-2单克隆抗体，通过间接免疫荧光法测量各自抗原的表达。用Ortho Cytofluorograph 50-H分析标记细胞的荧光。AP-2和AP-3的平均荧光强度（MFI）值结果显示，患者完全缺乏GPIIb-IIIa抗原。然而，患者的Mo-1以及对照Mo-2的表达（Mo-1：672和716；Mo-2：453和637）与正常受试者相似（Mo-1：735±74；Mo-2：585±35；平均值±标准误）。因此，血小板无力症患者单核细胞上Mo-1受体的正常表达表明，单核细胞上的Mo-1抗原和血小板上的GPIIb-IIIa复合物存在不同的基因组调控。
