Department of Neurology, Neuromuscular Molecular Pathology, University of Tampere, Biokatu 10, Finn-Medi 3, 33520 Tampere, Finland.
Acta Neuropathol. 2010 Apr;119(4):495-500. doi: 10.1007/s00401-010-0643-8. Epub 2010 Jan 28.
The different histochemical ATPase properties of myosins separating the muscle fiber types have been utilized in diagnostic muscle biopsy routine for more than four decades. The ATPase staining method is rather laborious and has several disadvantages, such as weakening of staining over time and non-specific staining of capillaries, making the distinction of extremely atrophic muscle fibers difficult. We have developed a reliable and advanced immunohistochemical myosin double staining method for the identification of fiber types, including highly atrophic fibers in routine diagnostics. With this double staining method, we are able to distinguish among type I (ATPase type 1), IIA (ATPase type 2A), IIX (ATPase type 2B) and remodeled ATPase type 2C fibers expressing both fast and slow myosins using a one slide technique. Immunohistochemical double staining of myosin heavy chain isoforms can be used as an alternative for the conventional ATPase staining method in routine histopathology. The method provides even more detailed information of fast fiber subtypes and highly atrophic fibers on one single slide.
不同肌球蛋白的组织化学 ATP 酶特性将肌纤维类型分离，这一方法在诊断性肌肉活检常规中已经使用了四十多年。ATP 酶染色方法相当繁琐，有几个缺点，例如随着时间的推移染色减弱和毛细血管的非特异性染色，使得极度萎缩的肌纤维的区分变得困难。我们已经开发出一种可靠和先进的免疫组织化学肌球蛋白双重染色方法，用于识别纤维类型，包括常规诊断中的高度萎缩纤维。使用这种双重染色方法，我们能够使用一种载玻片技术区分 I 型（ATPase 类型 1）、IIA 型（ATPase 类型 2A）、IIX 型（ATPase 类型 2B）和表达快、慢肌球蛋白的重塑型 ATPase 类型 2C 纤维。肌球蛋白重链同工型的免疫组织化学双重染色可作为常规组织病理学中传统 ATP 酶染色方法的替代方法。该方法在一张载玻片上提供了关于快纤维亚型和高度萎缩纤维的更详细信息。
