Department of Gynaecology and Obstetrics, First Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Arch Gynecol Obstet. 2011 Feb;283(2):317-21. doi: 10.1007/s00404-010-1366-8. Epub 2010 Jan 28.
To explore the role of mother's peripheral blood mononuclear cell (PBMC) infected with hepatitis B virus (HBV) in intrauterine transmission.
We have selected 60 cases of pregnant women with negative serum HBV DNA and positive PBMC HBV DNA from hospitalized patients. These women and their neonates acted as the experimental group. Twenty cases of pregnant women with HBV serum marker negative were selected. These women and their neonates served as the control group. Immunohistochemistry was employed to detect the expressions of HBsAg and HBcAg in cells of every placental layer and CD68 cells of placenta of the pregnant women whose neonates' PBMC HBV DNA was positive and/or whose neonates' serum HBV DNA positive.
In the experimental group, neonatal serum HBV DNA of only four cases were positive, only eight cases' neonatal PBMC HBV DNA were positive and four cases had HBV DNA positive in both neonatal serum and PBMC. The expressions of HBsAg and HBcAg were detected in CD68 cells of villous stroma and blood capillary in only eight cases of neonatal placenta with positive PBMC HBV DNA. HBV infection was found in cells of every layer in placenta in two of four cases with neonatal serum HBV DNA positive. The expressions of HBsAg and HBcAg were detected in trophoblastic cells, CD68 cells of villous stroma and blood capillary in two of four cases with HBV DNA positive in both neonatal serum and PBMC. In control group, no positive signals were detected in neonates and placenta.
HBV-infected PBMC in pregnant women may lead to intrauterine infection.
探讨乙型肝炎病毒（HBV）感染母亲外周血单个核细胞（PBMC）在宫内传播中的作用。
选取我院住院患者中乙型肝炎病毒血清学标志物阴性、PBMC HBV DNA 阳性的孕妇 60 例及其新生儿为实验组，选择乙型肝炎病毒血清学标志物阴性的孕妇 20 例及其新生儿为对照组。采用免疫组化法检测新生儿 PBMC HBV DNA 阳性和/或新生儿血清 HBV DNA 阳性孕妇胎盘各层细胞中 HBsAg 和 HBcAg 及胎盘 CD68 细胞的表达。
实验组中仅 4 例新生儿血清 HBV DNA 阳性，8 例新生儿 PBMC HBV DNA 阳性，4 例新生儿血清和 PBMC 均 HBV DNA 阳性。仅 8 例 PBMC HBV DNA 阳性新生儿胎盘的绒毛间质和毛细血管 CD68 细胞中检测到 HBsAg 和 HBcAg 的表达。4 例新生儿血清 HBV DNA 阳性中有 2 例胎盘各层细胞均检测到 HBV 感染，4 例新生儿血清和 PBMC 均 HBV DNA 阳性中有 2 例胎盘滋养细胞、绒毛间质和毛细血管 CD68 细胞中检测到 HBsAg 和 HBcAg 的表达。对照组新生儿和胎盘均未检测到阳性信号。
HBV 感染孕妇的 PBMC 可能导致宫内感染。
