矿物油中的过氧化物用于体外受精：定义标准质量控制检测的极限。
Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays.
机构信息
Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Rochester, MN, USA.
出版信息
J Assist Reprod Genet. 2010 Feb;27(2-3):87-92. doi: 10.1007/s10815-009-9383-x. Epub 2010 Jan 27.
Abstract
PURPOSE
To determine the relative sensitivities of the 1 and 2-cell mouse embryo assays (MEA) and the human sperm motility assay (HSMA) for peroxides in mineral oil. The effect of peroxide on blastocyst cell number and apoptosis was also studied.
METHODS
One and two-cell MEA and HSMA were performed using mineral oil containing cumene hydroperoxide (CH).
RESULTS
The 1-cell MEA was twice as sensitive as the 2-cell MEA and 20-times more sensitive than the HSMA for CH in mineral oil. The sensitivity of the 1-cell MEA doubled when embryos were cultured individually versus group culture. CH decreased blastocyst cell number in a dose dependent manner.
CONCLUSIONS
Individually cultured 1-cell embryos had the highest sensitivity for peroxides in mineral oil. Current quality control assays, including group cultured murine embryos and human sperm motility, have limited sensitivity for peroxides in mineral oil and may not detect levels of peroxides that cause sub-lethal cellular damage.
摘要
目的
确定矿物油中过氧化物在 1 细胞和 2 细胞小鼠胚胎检测（MEA）以及人精子活力检测（HSMA）中的相对灵敏度。还研究了过氧化物对囊胚细胞数量和细胞凋亡的影响。
方法
使用含有过氧化枯烯（CH）的矿物油进行 1 细胞和 2 细胞 MEA 以及 HSMA。
结果
1 细胞 MEA 对矿物油中 CH 的灵敏度是 2 细胞 MEA 的两倍，是 HSMA 的 20 倍。当胚胎单独培养而不是分组培养时，1 细胞 MEA 的灵敏度增加了一倍。CH 呈剂量依赖性降低囊胚细胞数量。
结论
单独培养的 1 细胞胚胎对矿物油中的过氧化物具有最高的灵敏度。目前的质量控制检测，包括分组培养的小鼠胚胎和人精子活力，对矿物油中的过氧化物的灵敏度有限，并且可能无法检测到引起亚致死性细胞损伤的过氧化物水平。
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