海兔钾离子通道的克隆与表达及其与海兔天然钾离子电流的比较。
Cloning and expression of an Aplysia K+ channel and comparison with native Aplysia K+ currents.
作者信息
Pfaffinger P J, Furukawa Y, Zhao B, Dugan D, Kandel E R
机构信息
Howard Hughes Medical Institute, Department of Physiology and Biophysics, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
出版信息
J Neurosci. 1991 Apr;11(4):918-27. doi: 10.1523/JNEUROSCI.11-04-00918.1991.
We describe here the cloning of the Aplysia K+ channel AK01a.AK01a codes for a protein of 515 amino acids, shows considerable homology to other cloned potassium channels, and can be classified as a member of the ShakerK+ channel family. Expression of the AK01a channel in Xenopus oocytes produces a rapidly inactivating outward potassium current (IAK01a) resembling the A-type currents of Drosophila Shaker. Gating for this current is shifted to potentials considerably more positive than the traditional A-currents of Aplysia; we have, however, identified a novel transient potassium current (IAdepoI) in a subset of Aplysia neurons that has similar gating and pharmacological properties to IAK01a.
我们在此描述海兔钾通道AK01a的克隆。AK01a编码一个由515个氨基酸组成的蛋白质，与其他已克隆的钾通道有相当高的同源性，可被归类为Shaker钾通道家族的一员。AK01a通道在非洲爪蟾卵母细胞中的表达产生一种快速失活的外向钾电流（IAK01a），类似于果蝇Shaker的A 型电流。该电流的门控电位比海兔传统的A电流正得多；然而，我们在一部分海兔神经元中发现了一种新型的瞬时钾电流（IAdepoI），其门控和药理学特性与IAK01a相似。
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