Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Dev Dyn. 2010 May;239(5):1352-64. doi: 10.1002/dvdy.22226.
The invariant cell division patterns that characterize Caenorhabditis elegans development make it an ideal system to study the mechanisms that control nuclear movement and positioning. Forward genetic screens in this system allowed identification of the key molecular machinery for connecting the nucleus to the cytoskeleton; pairs of protein partners, consisting of a KASH domain protein and a SUN domain protein, bridge the nuclear envelope to connect the nucleus to cytoskeletal components. The C. elegans genome encodes several KASH/SUN pairs, and mutant phenotypes as well as tissue-specific expression patterns suggest a diversity of functions. These functions include moving the nucleus but have been extended to effects on the chromosomes inside the nucleus as well. We review the impact of the C. elegans system in pioneering this field as well as the functions of these KASH/SUN protein pairs across spatial and temporal C. elegans development.
秀丽隐杆线虫的细胞分裂模式具有不变性，这使其成为研究控制核运动和定位机制的理想系统。该系统中的正向遗传筛选鉴定出了将核与细胞骨架连接的关键分子机制；由 KASH 结构域蛋白和 SUN 结构域蛋白组成的蛋白对将核膜桥接到连接核与细胞骨架成分的位置。秀丽隐杆线虫基因组编码几种 KASH/SUN 对，突变表型和组织特异性表达模式表明其具有多样性的功能。这些功能包括移动核，但也已经扩展到对核内染色体的影响。我们综述了秀丽隐杆线虫系统在开拓这一领域的影响，以及这些 KASH/SUN 蛋白对在秀丽隐杆线虫时空发育过程中的功能。
