School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia.
Dev Dyn. 2010 Mar;239(3):865-74. doi: 10.1002/dvdy.22221.
pbx1, a TALE (three-amino acid loop extension) homeodomain transcription factor, is involved in a diverse range of developmental processes. We examined the expression of pbx1 during zebrafish development by in situ hybridization. pbx1 transcripts could be detected in the central nervous system and pharyngeal arches from 24 hpf onwards. In the swim bladder anlage, pbx1 was detected as early as 28 hpf, making it the earliest known marker for this organ. Morpholino-mediated gene knockdown of pbx1 revealed that the swim bladder failed to inflate, with eventual lethality occurring by 8 dpf. The knockdown of pbx1 did not perturb the expression of prdc and foxA3, with both early swim bladder markers appearing normally at 36 and 48 hpf, respectively. However, the expression of anxa5 was completely abolished by pbx1 knockdown at 60 hpf suggesting that pbx1 may be required during the late stage of swim bladder development.
pbx1 是一个 TALE（三氨基酸环延伸）同源域转录因子，参与多种发育过程。我们通过原位杂交技术检查了 pbx1 在斑马鱼发育过程中的表达。从 24 hpf 开始，pbx1 转录本可以在中枢神经系统和咽弓中检测到。在鳔原基中，早在 28 hpf 就检测到了 pbx1，使其成为该器官最早的已知标记物。pbx1 的 morpholino 介导的基因敲低显示鳔未能充气，最终在 8 dpf 时发生致死。pbx1 的敲低并没有扰乱 prdc 和 foxA3 的表达，这两个早期鳔标记物分别在 36 和 48 hpf 时正常出现。然而，在 60 hpf 时，pbx1 的敲低完全消除了 anxa5 的表达，表明 pbx1 可能在鳔发育的晚期阶段是必需的。
